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WB analysis using Anti-GH antibody (A00851-2) in mouse hippocampal tissue revealed a specific band at the expected molecular weight with negligible non-specific signals.

Excellent, submitted by on
SKU A00851-2
Application Western Blot
Sample mouse hippocampal tissue
Sample Processing Description Total protein was extracted from the left hippocampus of normal mouse brain.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody GH1 Antibody Picoband®
Primary Incubation 1:4000, overnight at 4 ℃
Secondary Antibody HRP-conjugated goat anti-rabbit IgG
Secondary Incubation 1:10000, 1h in RT
Detection Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary Growth hormone (GH) serves as a central integrative signal that coordinates growth, metabolism, and tissue repair in response to changes in nutritional status. It is not only the primary driver of linear growth during puberty but also plays a critical role throughout life in maintaining muscle mass, bone strength, and metabolic flexibility. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the GH antibody. The results showed a band at the expected position with good specificity, indicating that the antibody performs well in WB applications.

The ELAVL1 antibody (A00736-1) produced a clear specific band at the expected size in WB using rat colon and colon cancer tissue samples, consistent with the expected expression pattern.

Excellent, submitted by on
SKU A00736-1
Application Western Blot
Sample HepG2 subcutaneous xenograft in nude mice
Sample Processing Description ① normal rat colon tissue; ② rat colon cancer model tissue; total protein extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody HuR/ELAVL1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP-conjugated goat anti-rabbit IgG
Secondary Incubation 1:10000, 30 min in 37℃
Detection Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary HUR protein is an RNA-binding protein that plays multiple key roles in gene expression regulation. It is essential in maintaining cellular homeostasis, stress response, inflammation, and the development and progression of diseases, especially cancer. In normal tissues, it is expressed at moderate levels, maintaining a delicate balance of cell proliferation, stress response, inflammation, and neural functions, while in tumors it is highly overexpressed, driving malignant progression. The experimental results are consistent with these observations.

WB results showed that Cingulin/CGN (A02373-1) was downregulated in the rat colon model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by on
SKU A02373-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents5% non-fat milk
Primary Antibody Cingulin/CGN Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of Cingulin/CGN and the internal control Actin in rat colon across different groups; expression was decreased in the model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by on
SKU M01394-4
Application Immunofluorescence
Sample mouse brain tissue
Sample Processing Description Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other ReagentsGoat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DMi3000
Results Summary IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.

One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

Excellent, submitted by on
SKU DZ41739
Application Western Blot
Sample egg lysate
Sample Processing Description Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC581356 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Excellent, submitted by on
SKU DZ41738
Application Western Blot
Sample Embryo lysate
Sample Processing Description Embryonic lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC584590 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.

Excellent, submitted by on
SKU DZ41648
Application Western Blot
Sample 3T3 Cell lysate
Sample Processing Description Nuclear and Cytoplasmic Extraction was performed on 3T3 cells with NE-PER kit by following the protocol and by adding protease and phosphatase inhibitors. Total protein was quantified with Qubit Protein Assay. Lysates were mixed with 4× Laemmli Sample Buffer and 2-mercaptoethanol, and then heated at 95°C for 5 min.
Primary Antibody Anti-Mouse Atxn7 Antibody
Primary Incubation Incubated in non-fat dry milk in TBST overnight at 4°C with antibody dilution at 1:1000 dilution
Secondary Antibody Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Secondary Incubation 1:4000 dilution in milk for 1 hour at room temperature
Other Reagents used SuperSignal West Pico PLUS Chemiluminescent Substrate
Detection Biorad ChemiDoc
Results Summary I was looking to target Atxn7 isoform 2, named as Atxn7b in the literature, and referenced as ENSMUST00000223714.2 on Ensembl. (It differs only on the C-terminus compared to Atxn7a, isoform 1). Atxn7 is mainly in the nucleus and is between 100-130 kDa. An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.