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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample HEK293T
Blocking Agent 5% Non-fat milk
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Azure Biosystems c600, ECL substrate
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Excellent CD14 ELISA Kit From BosterBio for Barrier Dysfunction

Excellent, submitted by on

Source: Biocompare.com

SKU EK0695
Application ELISA
Starting Material Serum
Tips Follow the incubation times closely
Results Summary Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS.

"CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction."

Good Mouse IL-12 ELISA Kit

--Daisy Dai University of Miami Surgery Lab Director / Manager

Excellent, submitted by on

Source: Biocompare.com

SKU EK0422
Application ELISA
Starting Material Crispr MEK and STAT3 KO CAFs
Tips This kit doesn't provide PC

"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."

No Complaints. A Simple, Effective ELISA from BosterBio

--David Nelson, University of Wisconsin, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU EK1458
Application ELISA
Sample Mouse plasma (with EDTA as anticoagulant)
Detection --

"Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays. 10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad. Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% - but consistent with the product literature. I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37°C) because I didn't want to deal with going back and forth into the room that houses our 37°C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image, Columns A and B are the standard curve." (Customer’s Tip: "100-fold dilution of mouse plasma resulted in all data falling on standard curve.")

Very Efficient ELISA Kit from BosterBio

--Abdullah Mamun, University of Texas Health Science Center at Houston, Neurology, Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU EK1185
Application ELISA
Sample Serum
Detection --

"Good sensitivity. I have found detectable levels of CD200. Overall, it is a very good kit for CD200 detection." (Customer’s Tip: "I have used neat samples.")

Great TNF-Alpha Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

SKU EK0527
Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")

Great IL-6 Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

SKU EK0411
Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")