| SKU | A03213-2 |
|---|---|
| Application | Western Blot |
| Sample | MCF-7 cell |
| Sample Processing Description | Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE. |
| Primary Antibody | UBA6 Antibody |
| Primary Incubation | 1:1000, overnight at 4 °C |
| Blocking Agent | 5% Non-fat milk |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Tanon system. |
| Results Summary | The antibody performs efficiently and specifically, with very few nonspecific bands. |