• Compare Products

  • Table of Contents

Customer Testimonials

Peer Reviews from Real Labs

Browse peer-submitted results linked to specific products—ratings, images, and practical notes included. Contribute your own result to strengthen the evidence base and claim rewards after verification.

Share Your Review Browse Reviews ↓

Video Interviews

See real workflows and decision points—what researchers tried, what worked, and why. Share your story in a short interview and claim rewards!

Get Interview Rewards

Filter by category, reactivity, and host to find the most relevant peer results.

Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loa

Excellent, submitted by on
SKU DZ41317
Application Western Blot
Sample Drosophila Ovaries
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Anti-Fruit fly Phb2 Antibody
Primary Incubation 1:1000 in 5% BSA and 0.1% PBST. Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody 1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1 hour at room temperature
Detection Biorad ChemiDoc
Results Summary Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loading.

Drosophila fatbody showed good staining of Phb2.

Excellent, submitted by on
SKU DZ41317
Application Immunofluorescence
Sample Drosophila Fat body and Ovaries
Sample Processing Description Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody Anti-Fruit fly Phb2 Antibody
Primary Incubation 1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody 1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation 1 hour at room temperature
Detection Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary Drosophila fatbody showed good staining of Phb2.

This was only optimization experiment and still needs validation through necessary experiments.

Excellent, submitted by on
SKU DZ41315
Application Immunofluorescence
Sample Drosophila Fat body and Ovaries
Sample Processing Description Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody Anti-Fruit fly Marf Antibody
Primary Incubation 1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody 1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation 1 hour at room temperature
Detection Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary This was only optimization experiment and still needs validation through necessary experiments.

Antibody Optimized and validated

Excellent, submitted by on
SKU DZ41314
Application Western Blot
Sample Drosophila Ovaries
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Anti-Fruit Fly Drp1 Antibody
Primary Incubation 1:1000 in 5% BSA and 0.1% PBST Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody 1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1 hour at room temperature
Other Reagents used ECL Clarity Substrate and enzyme for Chemiluminescence
Detection Biorad ChemiDoc
Results Summary In Drosophila ovaries, loss of Drp1 function was confirmed. Western blot analysis validated Drp1 knockdown, showing reduced Drp1 protein levels (~85 kDa) in ovaries expressing Drp1 miRNA, with β-Actin serving as a loading control.

Antibody Optimized and validated

Excellent, submitted by on
SKU DZ41314
Application Immunofluorescence
Sample Drosophila Fat body
Sample Processing Description Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes, Secondary, Washes, Nuclei Staining, Slide Preparation
Primary Antibody Anti-Fruit Fly Drp1 Antibody
Primary Incubation 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody 1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation 1 hour at room temperature
Detection Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary In Drosophila fatbody, Drp1 was seen as punctate structures, as expected from the literature on mitochondria.

Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Excellent, submitted by on
SKU DZ41119
Application Immunofluorescence
Sample Zebrafish retinal cryo-section
Sample Processing Description Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm
Primary Antibody Zebrafish Tfap2a Antibody
Primary Incubation 1:100, overnight at 4 ℃
Detection Used a Nikon C2+ confocal microscope
Results Summary Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Thank you for all the work on this project

Excellent, submitted by on
SKU DZ01481
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:200
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in blocking solution.

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Excellent, submitted by on
SKU DZ4103
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution.

Both are confirmed specific in zebrafish using one of our KO lines.

Excellent, submitted by on
SKU DZ41032
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution.

Beautiful Imaging of Zebrafish Eosinophils

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41383
Application Immunofluorescence
Sample Zebrafish WKM, IPEX and FFPE tissue sections
Primary Incubation Overnight
Blocking Agent 3% goat serum in PBS
Secondary Incubation 1 hour
Tertiary Incubation None
Detection Fluorescence microscopy
DOI or PMID # https://doi.org/10.1101/2024.04.29.591640
Results Summary Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF.

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."