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Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by on
SKU M01394-4
Application Immunofluorescence
Sample mouse brain tissue
Sample Processing Description Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other ReagentsGoat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DMi3000
Results Summary IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.

In this study, this antibody was mainly used for Western Blot experiments. In the Western Blot results, the CCT3 antibody showed a clear and specific target band and maintained a strong signal even after multiple rounds of reuse.

Excellent, submitted by on
SKU PB9926
Application Western Blot
Sample U2OS cells
Primary Incubation 1:1000
Blocking Agent BSA
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.