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In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by Yu Lei, School of Life Sciences, Wuhan University on 2026-02-26

SKU	M01394-4
Application	Immunofluorescence
Sample	mouse brain tissue
Sample Processing Description	Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other Reagents	Goat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody	Iba1 Rabbit Monoclonal Antibody
Primary Incubation	1:100, overnight at 4 ℃
Secondary Antibody	Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DMi3000
Results Summary	IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.