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WB analysis using Anti-CDK2 antibody (M00166) in mouse hippocampal tissue showed a specific band at the expected size with low background, indicating good performance.

Excellent, submitted by on
SKU M00166
Application Western Blot
Sample mouse hippocampal tissue
Sample Processing Description Total protein was extracted from the left hippocampus of normal mouse brain.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody CDK2 Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated goat anti-rabbit IgG
Secondary Incubation 1:10000, 1 hour in RT
Detection Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary CDK2 (cyclin-dependent kinase 2) plays a central role in driving the cell cycle and serves as a key node integrating proliferative signaling, DNA damage response, and chemotherapy stress. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the CDK2 antibody. The results showed a specific band at the expected molecular weight with good signal quality, indicating reliable antibody performance.

WB analysis using Anti-ATP1A1 antibody (M00956) in HepG2 cells showed a clear and specific band at the expected molecular weight, with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M00956
Application Western Blot
Sample human adrenocortical carcinoma tissue
Sample Processing Description Total protein was extracted from clinically collected human adrenocortical carcinoma tissue.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP-conjugated goat anti-rabbit IgG
Secondary Incubation 1:10000, 1h in RT
Detection Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary ATP1A1 encodes the α1 catalytic subunit of the Na⁺/K⁺-ATPase, whose core physiological function is to maintain the transmembrane sodium and potassium electrochemical gradient, serving as the basis for nearly all vital processes such as neuronal excitability, renal reabsorption, and muscle contraction. In this study, normally cultured HepG2 cells (without any drug treatment) were used to validate the quality of the ATP1A1 antibody. The results showed a clear target band at the correct position with clean background, indicating that the antibody performs well.

The Anti-FOXO3A antibody (M00252) produced clear, specific bands with low background, and reliably detected expected changes in FOXO3A levels in normal, Alzheimer’s model, and treated mouse hippocampal tissues, demonstrating excellent performance.

Excellent, submitted by on
M00252 wb
SKU M00252
Application Western Blot
Sample mouse brain tissue
Sample Processing Description ① Normal mouse hippocampal tissue, ② Hippocampal tissue from Alzheimer’s disease model mouse, ③ Hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug. Total protein was extracted from all samples.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody TNF alpha Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary FOXO3a is a key member of the FOXO transcription factor family, playing a central regulatory role in cellular processes such as stress response, apoptosis, autophagy, metabolism, and antioxidation. In Alzheimer’s disease, FOXO3a is known to exert neuroprotective effects. Experimental results show that FOXO3a protein levels are significantly decreased in the brains of Alzheimer’s model mice, while treatment leads to a noticeable increase.

E-cadherin Antibody Picoband® (PB9561) shows clear, specific bands in human cervical cancer and adjacent tissues by WB, with higher expression in adjacent tissues than in cancer tissues, demonstrating excellent antibody performance.

Excellent, submitted by on
PB9561 wb
SKU PB9561
Application Western Blot
Sample human cervical cancer and adjacent tissues
Sample Processing Description Three samples each of human cervical cancer and adjacent tissues were collected, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody E Cadherin 1/CDH1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary Based on this study, the expression of E-cadherin in human cervical cancer and adjacent tissues was compared, and results from three cases showed that E-cadherin levels were significantly higher in adjacent tissues than in cancer tissues.

MTOR Antibody (M00003) WB on rat skeletal muscle shows clear bands, with higher MTOR in the model and dose-dependent reduction in treatment groups.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Total protein was extracted from rat skeletal muscle tissue: ① normal, ② injury model, ③ high-dose drug treatment, ④ medium-dose drug treatment, ⑤ low-dose drug treatment.
Other ReagentsRIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The results show clear MTOR bands, with higher levels in the injury model compared to normal, and a dose-dependent decrease in the treatment groups, as expected.

The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by on
SKU M01072-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate
Imaging system: ChemiDoc MP
Results Summary This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M00344
Application Western blot
Sample human cervical cancer paraffin sections
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody CD4 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.