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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by on
SKU MA1045
Application Immunofluorescence
Sample rat spinal tissue
Sample Processing Description Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 minutes in 37℃
Detection Imaging system:Leica DM2500
Results Summary GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.

This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU PB9475
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 4/AQP4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory.

Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Excellent, submitted by on
SKU DZ41119
Application Immunofluorescence
Sample Zebrafish retinal cryo-section
Sample Processing Description Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm
Primary Antibody Zebrafish Tfap2a Antibody
Primary Incubation 1:100, overnight at 4 ℃
Detection Used a Nikon C2+ confocal microscope
Results Summary Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Excellent, submitted by on
SKU PA1050
Application Immunofluorenscence
Sample Mouse spinal cord
Sample Processing Description Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody Anti-MBP9 antibody
Primary Incubation 1:100, overnight at 4 ℃
Blocking Agent 3% BSA
Secondary Antibody Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Excellent, submitted by on
SKU DZ4103
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution.

Both are confirmed specific in zebrafish using one of our KO lines.

Excellent, submitted by on
SKU DZ41032
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution.

Beautiful Imaging of Zebrafish Eosinophils

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41383
Application Immunofluorescence
Sample Zebrafish WKM, IPEX and FFPE tissue sections
Primary Incubation Overnight
Blocking Agent 3% goat serum in PBS
Secondary Incubation 1 hour
Tertiary Incubation None
Detection Fluorescence microscopy
DOI or PMID # https://doi.org/10.1101/2024.04.29.591640
Results Summary Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF.

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."

The antibody worked well even though we used fresh frozen brain sections cut at 20 micron thickness thaw-mounted onto microscope slide that were then post-fixed with 4% paraformaldehyde. The antibody worked equally well when used on fixed brains sectioned

Excellent, submitted by on

Boster bio Iba1 1:100 PFA (3-4) - cortex 12x - with scale bar

Boster bio Iba1 1:100 PFA (3-4) - cortex 25x - with scale bar

SKU A01394
Application Immunofluorescence
Sample Mouse brain
Primary Antibody Dilution 1:100

Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of Iba1 (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.