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| SKU | M30929 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | Anti-Rabbit IgG Secondary Antibody, HRP-conjugated |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Azure Biosystems c600. |
| Results Summary | I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues. |
Source: Biocompare.com
| SKU | DZ33980 |
|---|---|
| Application | Western Blot |
| Sample | Recombinant protein expressed in and purified from E. coli |
| Detection | Chemiluminescence |
"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."