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Peer Reviews from Real Labs

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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample HEK293T
Blocking Agent 5% Non-fat milk
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Azure Biosystems c600, ECL substrate
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Successful MMP-9 measurements

Excellent, submitted by on

Source: Biocompare.com

SKU EK0465
Application Test the quantity of MMP-9 released from neutrophils after challenge with bacteria
Starting Material Human Neutrophils
Protocol Overview 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader
Tips Frozen supernatants work just as well as fresh ones
Results Summary I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."

User-Friendly & Reliable AGP1 ELISA Kit

Excellent, submitted by on

Source: Biocompare.com

SKU EK1486
Application Urinary Biomarker Discovery
Starting Material Urine
Tips Fast and good product.
Results Summary Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties.

"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."

Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well

Excellent, submitted by on

Source: Biocompare.com

SKU EK7098
Application Human TSH in dried blood spots
Starting Material Dried blood spots
Tips Use the 0 standard as a diluent, PBS works decently if not.
Results Summary The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range.

"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."

Reliable Human T3 ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7010
Application Human progesterone quantification in dried blood spots
Starting Material Dried Blood Spots
Tips Use the 0 standard as a diluent, don't include it on the standard curve.
Results Summary Our data concluded that dried blot spots can be used to quantify T3 levels in humans. The sensitivity remains relevant. Results are consistent and repeatable.

"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."

Superb Human Progesterone ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7004
Application Human Progesterone saliva levels
Starting Material Saliva
Tips This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop.
Results Summary This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity.

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."

Human T4 ELISA Kit from Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7012
Application Human T4 levels in Dried Blood Spots
Starting Material Dried Blood Spots
Tips This is a competitive ELISA without a 0 standard or a diluent, PBS makes a reliable diluent for patient samples and standards.
Results Summary Results were repeatable and reliable using dried blood spots. The highest concentration standard sometimes fell OWR, but the low end stayed within reading capabilities.

"The goal of this experiment is to quantify the levels of human T4 in dried blood spots from at-home test kits. This assay in combination with other hormone tests is aimed at providing accurate and timely healthcare to patients remotely."