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Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In this study, this antibody was mainly used for Western Blot experiments. In the Western Blot results, the CCT3 antibody showed a clear and specific target band and maintained a strong signal even after multiple rounds of reuse.

Excellent, submitted by on
SKU PB9926
Application Western Blot
Sample U2OS cells
Primary Incubation 1:1000
Blocking Agent BSA
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Excellent CD14 ELISA Kit From BosterBio for Barrier Dysfunction

Excellent, submitted by on

Source: Biocompare.com

SKU EK0695
Application ELISA
Starting Material Serum
Tips Follow the incubation times closely
Results Summary Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS.

"CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction."

Successful MMP-9 measurements

Excellent, submitted by on

Source: Biocompare.com

SKU EK0465
Application Test the quantity of MMP-9 released from neutrophils after challenge with bacteria
Starting Material Human Neutrophils
Protocol Overview 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader
Tips Frozen supernatants work just as well as fresh ones
Results Summary I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."

A New and Reliable ELISA for Rat FABP4

Excellent, submitted by on

Source: Biocompare.com

SKU EK1573
Application ELISA
Starting Material Rat Plasma
Tips Kit suggested sample dilution is 1:50 but I used 1:25 and got results within standard range.
Results Summary We compared plasma FABP4 levels in pregnant and nonpregnant rats and results were consistent with reported studies.

"There were a number of ELISA kits for human and mice FABP4 but rat-specific kits were not easily available. We tried this Kit from Boster and are happy with the kit performance. The steps were easy to follow and standers and sample values were in an acceptable range."

User-Friendly & Reliable AGP1 ELISA Kit

Excellent, submitted by on

Source: Biocompare.com

SKU EK1486
Application Urinary Biomarker Discovery
Starting Material Urine
Tips Fast and good product.
Results Summary Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties.

"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."

Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well

Excellent, submitted by on

Source: Biocompare.com

SKU EK7098
Application Human TSH in dried blood spots
Starting Material Dried blood spots
Tips Use the 0 standard as a diluent, PBS works decently if not.
Results Summary The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range.

"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."

Reliable Human T3 ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7010
Application Human progesterone quantification in dried blood spots
Starting Material Dried Blood Spots
Tips Use the 0 standard as a diluent, don't include it on the standard curve.
Results Summary Our data concluded that dried blot spots can be used to quantify T3 levels in humans. The sensitivity remains relevant. Results are consistent and repeatable.

"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."

Superb Human Progesterone ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7004
Application Human Progesterone saliva levels
Starting Material Saliva
Tips This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop.
Results Summary This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity.

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."