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Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In this study, this antibody was mainly used for Western Blot experiments. In the Western Blot results, the CCT3 antibody showed a clear and specific target band and maintained a strong signal even after multiple rounds of reuse.

Excellent, submitted by on
SKU PB9926
Application Western Blot
Sample U2OS cells
Primary Incubation 1:1000
Blocking Agent BSA
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Excellent CD14 ELISA Kit From BosterBio for Barrier Dysfunction

Excellent, submitted by on

Source: Biocompare.com

SKU EK0695
Application ELISA
Starting Material Serum
Tips Follow the incubation times closely
Results Summary Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS.

"CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction."

Good Mouse IL-12 ELISA Kit

--Daisy Dai University of Miami Surgery Lab Director / Manager

Excellent, submitted by on

Source: Biocompare.com

SKU EK0422
Application ELISA
Starting Material Crispr MEK and STAT3 KO CAFs
Tips This kit doesn't provide PC

"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."

No Complaints. A Simple, Effective ELISA from BosterBio

--David Nelson, University of Wisconsin, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU EK1458
Application ELISA
Sample Mouse plasma (with EDTA as anticoagulant)
Detection --

"Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays. 10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad. Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% - but consistent with the product literature. I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37°C) because I didn't want to deal with going back and forth into the room that houses our 37°C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image, Columns A and B are the standard curve." (Customer’s Tip: "100-fold dilution of mouse plasma resulted in all data falling on standard curve.")

Very Efficient ELISA Kit from BosterBio

--Abdullah Mamun, University of Texas Health Science Center at Houston, Neurology, Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU EK1185
Application ELISA
Sample Serum
Detection --

"Good sensitivity. I have found detectable levels of CD200. Overall, it is a very good kit for CD200 detection." (Customer’s Tip: "I have used neat samples.")

Great TNF-Alpha Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

SKU EK0527
Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")

Great IL-6 Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

SKU EK0411
Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")

It is a good kit with good quality.

--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE

Excellent, submitted by on

Source: Biocompare.com

SKU EK0309
Applications ELISA
Sample Brain Tissue Lysate
Detection --

"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."