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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In WB using β-tubulin antibody (Cat# A01857-1), a clear, specific band was observed in OCI-LY8 cells with clean background, showing stable expression after SRPK1 knockdown.

Excellent, submitted by on
SKU A01857-1
Application Western Blot
Sample human OCI-LY1 cells
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Beta Tubulin/TUBB Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary The antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has clear and well-defined edges.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU M30929
Application Western Blot
Sample HEK293T
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent 5% non-fat milk
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Azure Biosystems c600.
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

The antibody performs efficiently and specifically, with very few nonspecific bands.

Excellent, submitted by on
WB result image
SKU A03213-2
Application Western Blot
Sample MCF-7 cell
Sample Processing Description Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE.
Primary Antibody UBA6 Antibody
Primary Incubation 1:1000, overnight at 4 °C
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Tanon system.
Results Summary The antibody performs efficiently and specifically, with very few nonspecific bands.

The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection.

Excellent, submitted by on
SKU DZ41720
Application Western Blot
Sample HEK293T whole cell lysates
Sample Processing Description HEK293T cells were transfected with a plasmid expressing SP only (and not Env or Rem) and lysed using RIPA buffer. A total of 80 µg of protein was resolved on a 4–12% gradient SDS-PAGE gel and transferred to a membrane. The membrane was blocked for 1 hour at room temperature with 5% non-fat milk in PBS-Tween (PBS-T). After washing three times with 1× PBS-T (10 minutes each), the membrane was incubated overnight at 4 °C with varying dilutions ofthe SP antibody prepared in 2% non-fat milk (1:500; 1:1000; 1:2000). Following three additional washes with PBS-T (10 minutes each), the membrane was incubated with anti-rabbit secondary antibody, washed again three times, and developed using ECL Plus.
Primary Incubation The membrane was incubated with the SP primary antibody (1:500; 1:1000; 1:2000) overnight at 4 °C.
Secondary Antibody Anti-rabbit-HRP-conjugated secondary antibody
Secondary Incubation Dilution: 1:25,000 in PBS-T/2% milk
Other Reagents used 1× RIPA lysis buffer
1× PBS-T washing buffer (T = 1% Tween 20)
Non-fat milk (for blocking and antibody dilution)
Anti-rabbit HRP-conjugated secondary antibody
Detection Signal was developed using ECL Plus chemiluminescent substrate
Results Summary The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection. Dilutions of 1:1000 and 1:2000 yielded the most optimal results, showing minimal background compared to 1:500, which highlights the antibody’s high specificity and sensitivity at these concentrations. To ensure equal protein loading across lanes, the membrane was also probed with a β-actin antibody, confirming equal sample loading and validating the observed signal intensity for SP. Overall, these results demonstrate that the SP antibody is reliable for detecting SP in RIPA-lysed HEK293T samples.

Immunohistochemistry for Anti-Mannose Receptor/MRC1

Excellent, submitted by on
SKU A02285-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse skin
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min