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Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by Ruibo Zhao, South China Agricultural University on 2026-01-26

SKU	MA1045
Application	Immunofluorescence
Sample	rat spinal tissue
Sample Processing Description	Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other Reagents	Tris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody	GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation	45 minutes in 37℃
Detection	Imaging system:Leica DM2500
Results Summary	GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.

Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loa

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41317
Application	Western Blot
Sample	Drosophila Ovaries
Sample Processing Description	Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody	Anti-Fruit fly Phb2 Antibody
Primary Incubation	1:1000 in 5% BSA and 0.1% PBST. Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody	1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation	1 hour at room temperature
Detection	Biorad ChemiDoc
Results Summary	Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loading.

Drosophila fatbody showed good staining of Phb2.

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41317
Application	Immunofluorescence
Sample	Drosophila Fat body and Ovaries
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit fly Phb2 Antibody
Primary Incubation	1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	Drosophila fatbody showed good staining of Phb2.

This was only optimization experiment and still needs validation through necessary experiments.

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41315
Application	Immunofluorescence
Sample	Drosophila Fat body and Ovaries
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit fly Marf Antibody
Primary Incubation	1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	This was only optimization experiment and still needs validation through necessary experiments.

Antibody Optimized and validated

Excellent, submitted by Kasturi Mitra on 2026-01-06

SKU	DZ41314
Application	Western Blot
Sample	Drosophila Ovaries
Sample Processing Description	Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody	Anti-Fruit Fly Drp1 Antibody
Primary Incubation	1:1000 in 5% BSA and 0.1% PBST Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody	1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation	1 hour at room temperature
Other Reagents used	ECL Clarity Substrate and enzyme for Chemiluminescence
Detection	Biorad ChemiDoc
Results Summary	In Drosophila ovaries, loss of Drp1 function was confirmed. Western blot analysis validated Drp1 knockdown, showing reduced Drp1 protein levels (~85 kDa) in ovaries expressing Drp1 miRNA, with β-Actin serving as a loading control.

Antibody Optimized and validated

Excellent, submitted by Kasturi Mitra on 2026-01-06

SKU	DZ41314
Application	Immunofluorescence
Sample	Drosophila Fat body
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes, Secondary, Washes, Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit Fly Drp1 Antibody
Primary Incubation	1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	In Drosophila fatbody, Drp1 was seen as punctate structures, as expected from the literature on mitochondria.

BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Excellent, submitted by Zhixiong Liu, Xiamen University on 2025-10-17

SKU	PA1050
Application	Immunofluorenscence
Sample	Mouse spinal cord
Sample Processing Description	Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody	Anti-MBP9 antibody
Primary Incubation	1:100, overnight at 4 ℃
Blocking Agent	3% BSA
Secondary Antibody	Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary	BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

The CLIPB4 antibody was efficiently used to detect CLIPB4 protein secreted in the mosquito hemolymph. The protein migrates at ~40 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ33989-1
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CLIPB4 knockdown mosquito hemolymph
Primary Incubation	Overnight at 4°C
Secondary Incubation	For 1 hour at room temperature
Secondary Antibody Dilution	1:3000
Detection	Clarity Max Western ECL substrate
Results Summary	Image showing the specificity of CLIPB4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPB4 gene.

The CTL4 antibody was efficiently used to detect CTL4 protein secreted in the mosquito hemolymph. The protein migrates at ~15 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ41074
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CTL4 knockdown mosquito hemolymph
Primary Incubation	+4°C overnight
Secondary Incubation	For 1 hour at room temperature
Tertiary Incubation	1:1000
Detection	Clarity Max western ECL substrate
Results Summary	Image showing the specificity of CTL4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CTL4 gene.

The CLIPA14 antibody was efficiently used to detect CLIPA14 protein secreted in the mosquito hemolymph. The protein migrates at ~59 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ41073
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CLIPA14 knockdown mosquito hemolymph
Primary Incubation	+4°C overnight
Secondary Incubation	For 1 hour at room temperature
Tertiary Incubation	1:3000
Detection	Clarity Max western ECL substrate
Results Summary	Image showing the specificity of CLIPA14 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPA14 gene.