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Why I Choose Boster's $600 Custom Antibody – Customer Interview

This Validation De-risks a lot of Downstream Workflows!

The $600 custom antibody service is good enough to recommend!

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More specific, lower background, and highly reproducible

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Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by David Lee on 2026-01-08

SKU	A01263
Application	Western Blot
Sample	Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description	Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody	Anti-beta Actin ACTB Antibody
Primary Incubation	1:1000, incubated overnight at 4°C.
Secondary Antibody	Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation	1:5000 dilution, 1 hour at room temperature.
Other Reagents used	5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection	Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary	Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loa

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41317
Application	Western Blot
Sample	Drosophila Ovaries
Sample Processing Description	Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody	Anti-Fruit fly Phb2 Antibody
Primary Incubation	1:1000 in 5% BSA and 0.1% PBST. Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody	1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation	1 hour at room temperature
Detection	Biorad ChemiDoc
Results Summary	Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loading.

Drosophila fatbody showed good staining of Phb2.

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41317
Application	Immunofluorescence
Sample	Drosophila Fat body and Ovaries
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit fly Phb2 Antibody
Primary Incubation	1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	Drosophila fatbody showed good staining of Phb2.

This was only optimization experiment and still needs validation through necessary experiments.

Excellent, submitted by Kasturi Mitra on 2026-01-07

SKU	DZ41315
Application	Immunofluorescence
Sample	Drosophila Fat body and Ovaries
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit fly Marf Antibody
Primary Incubation	1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	This was only optimization experiment and still needs validation through necessary experiments.

Antibody Optimized and validated

Excellent, submitted by Kasturi Mitra on 2026-01-06

SKU	DZ41314
Application	Western Blot
Sample	Drosophila Ovaries
Sample Processing Description	Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody	Anti-Fruit Fly Drp1 Antibody
Primary Incubation	1:1000 in 5% BSA and 0.1% PBST Either 2 hours at RT or Overnight at 4 Degrees
Secondary Antibody	1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation	1 hour at room temperature
Other Reagents used	ECL Clarity Substrate and enzyme for Chemiluminescence
Detection	Biorad ChemiDoc
Results Summary	In Drosophila ovaries, loss of Drp1 function was confirmed. Western blot analysis validated Drp1 knockdown, showing reduced Drp1 protein levels (~85 kDa) in ovaries expressing Drp1 miRNA, with β-Actin serving as a loading control.

Antibody Optimized and validated

Excellent, submitted by Kasturi Mitra on 2026-01-06

SKU	DZ41314
Application	Immunofluorescence
Sample	Drosophila Fat body
Sample Processing Description	Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes, Secondary, Washes, Nuclei Staining, Slide Preparation
Primary Antibody	Anti-Fruit Fly Drp1 Antibody
Primary Incubation	1:100 in 2%BSA and 0.1% PBS-TX for Fat body
Secondary Antibody	1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5
Secondary Incubation	1 hour at room temperature
Detection	Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary	In Drosophila fatbody, Drp1 was seen as punctate structures, as expected from the literature on mitochondria.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by Ziru Wang, Jilin University on 2025-10-10

SKU	A01263
Application	Western Blot
Sample	HEK293T
Blocking Agent	5% Non-fat milk
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Azure Biosystems c600, ECL substrate
Results Summary	I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

The CLIPB4 antibody was efficiently used to detect CLIPB4 protein secreted in the mosquito hemolymph. The protein migrates at ~40 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ33989-1
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CLIPB4 knockdown mosquito hemolymph
Primary Incubation	Overnight at 4°C
Secondary Incubation	For 1 hour at room temperature
Secondary Antibody Dilution	1:3000
Detection	Clarity Max Western ECL substrate
Results Summary	Image showing the specificity of CLIPB4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPB4 gene.

The CTL4 antibody was efficiently used to detect CTL4 protein secreted in the mosquito hemolymph. The protein migrates at ~15 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ41074
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CTL4 knockdown mosquito hemolymph
Primary Incubation	+4°C overnight
Secondary Incubation	For 1 hour at room temperature
Tertiary Incubation	1:1000
Detection	Clarity Max western ECL substrate
Results Summary	Image showing the specificity of CTL4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CTL4 gene.

The CLIPA14 antibody was efficiently used to detect CLIPA14 protein secreted in the mosquito hemolymph. The protein migrates at ~59 kDa in SDS-PAGE.

Excellent, submitted by Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut on 2025-04-08

SKU	DZ41073
Application	Western Blot
Sample	β-galactosidase gene knockdown mosquito hemolymph, CLIPA14 knockdown mosquito hemolymph
Primary Incubation	+4°C overnight
Secondary Incubation	For 1 hour at room temperature
Tertiary Incubation	1:3000
Detection	Clarity Max western ECL substrate
Results Summary	Image showing the specificity of CLIPA14 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPA14 gene.