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Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU M30929
Application Western Blot
Sample HEK293T
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent 5% non-fat milk
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Azure Biosystems c600.
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample HEK293T
Blocking Agent 5% Non-fat milk
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Azure Biosystems c600, ECL substrate
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection.

Excellent, submitted by on
SKU DZ41720
Application Western Blot
Sample HEK293T whole cell lysates
Sample Processing Description HEK293T cells were transfected with a plasmid expressing SP only (and not Env or Rem) and lysed using RIPA buffer. A total of 80 µg of protein was resolved on a 4–12% gradient SDS-PAGE gel and transferred to a membrane. The membrane was blocked for 1 hour at room temperature with 5% non-fat milk in PBS-Tween (PBS-T). After washing three times with 1× PBS-T (10 minutes each), the membrane was incubated overnight at 4 °C with varying dilutions ofthe SP antibody prepared in 2% non-fat milk (1:500; 1:1000; 1:2000). Following three additional washes with PBS-T (10 minutes each), the membrane was incubated with anti-rabbit secondary antibody, washed again three times, and developed using ECL Plus.
Primary Incubation The membrane was incubated with the SP primary antibody (1:500; 1:1000; 1:2000) overnight at 4 °C.
Secondary Antibody Anti-rabbit-HRP-conjugated secondary antibody
Secondary Incubation Dilution: 1:25,000 in PBS-T/2% milk
Other Reagents used 1× RIPA lysis buffer
1× PBS-T washing buffer (T = 1% Tween 20)
Non-fat milk (for blocking and antibody dilution)
Anti-rabbit HRP-conjugated secondary antibody
Detection Signal was developed using ECL Plus chemiluminescent substrate
Results Summary The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection. Dilutions of 1:1000 and 1:2000 yielded the most optimal results, showing minimal background compared to 1:500, which highlights the antibody’s high specificity and sensitivity at these concentrations. To ensure equal protein loading across lanes, the membrane was also probed with a β-actin antibody, confirming equal sample loading and validating the observed signal intensity for SP. Overall, these results demonstrate that the SP antibody is reliable for detecting SP in RIPA-lysed HEK293T samples.