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One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

Excellent, submitted by on
SKU DZ41739
Application Western Blot
Sample egg lysate
Sample Processing Description Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC581356 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Excellent, submitted by on
SKU DZ41738
Application Western Blot
Sample Embryo lysate
Sample Processing Description Embryonic lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC584590 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."