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- Table of Contents
FAP antibody (M00422) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IHC analysis of FAP1 using anti-FAP1 antibody (M00422).
FAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAP1 Antibody (M00422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAP1 Antibody (M00422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-FAP1 Rabbit Monoclonal Antibody
FAP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
FAP antibody was positively detected by WB in:
human U-87MG whole cell, rat skin tissue, mouse skin tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FAP antibody was positively detected by IHC in:
human breast cancer tissue, human colon cancer tissue, human esophageal squamous cell carcinoma tissue, human lung cancer tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-FAP1 Rabbit Monoclonal Antibody
View all FAP Antibodies
View all FAP Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AbF22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FAP1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
87.7 kDa
Cite This Product
Anti-FAP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00422)
Protocols
Western Blot protocol for FAP antibody
Immunohistochemistry (IHC) protocol for FAP antibody
Publications
Application:
Species:
Characteristics of 18F-FAPI-04 PET/CT in patients with peritoneal metastasis and to predict treatment efficacy, a head-to-head comparison with 18F-FDG PET/CT
Published: 2025-06-02
Journal: CANCER IMAGING
Application: IHC
Impact Factor: 3.50
Species: Human
PMID: 40457405
Case Report: FAP+ fibroblasts and SPP1+ macrophages in SMARCA2-deficient while SMARCA4-preserved poorly differentiated lung adenocarcinoma: two case reports and multi-omics analysis
Published: 2025-05-16
Journal: Frontiers in Immunology
Application: IHC
Impact Factor: 5.90
PMID: 40453096
DAB2+ macrophages support FAP+ fibroblasts in shaping tumor barrier and inducing poor clinical outcomes in liver cancer
Published: 2024-08-12
Journal: Theranostics
Application: IHC
Impact Factor: 12.40
Species: Human
PMID: 39239526
Delineating the early dissemination mechanisms of acral melanoma by integrating single-cell and spatial transcriptomic analyses
Published: 2023-12-08
Journal: Nature Communications
Application: IHC
Impact Factor: 16.60
Species: Human
PMID: 38065972
Down-regulation of COL1A1 inhibits tumor-associated fibroblast activation and mediates matrix remodeling in the tumor microenvironment of breast cancer
Published: 2023-11-30
Journal: Open Life Sciences
Application: WB
Impact Factor: 2.20
Species: Human
PMID: 38045487
Single-cell RNA sequencing reveals pro-invasive cancer-associated fibroblasts in hypopharyngeal squamous cell carcinoma
Published: 2023-10-18
Journal: Cell Communication and Signaling
Application: IHC
Impact Factor: 8.40
Species: Human
PMID: 37853464
Small Leucine-Rich Proteoglycan PODNL1 Identified as a Potential Tumor Matrix-Mediated Biomarker for Prognosis and Immunotherapy in a Pan-Cancer Setting
Published: 2023-07-22
Journal: CURRENT ISSUES IN MOLECULAR BIOLOGY
Application: IF
Impact Factor: 3.10
Species: Human
PMID: 37504302
The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes
Published: 2018-09-07
Journal: Journal of Cancer
Application: IHC,WB
Impact Factor: 3.25
Species: Human
PMID: 30271487
Review
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Submit A Review1 Review For Anti-FAP1 Rabbit Monoclonal Antibody
The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.
| SKU | M00422 |
|---|---|
| Application | Immunohistochemistry |
| Sample | human liver cancer |
| Sample Processing Description | Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin. |
| Other Reagents | Goat serum, DAB Chromogenic Solution |
| Primary Antibody | FAP1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC kit (Immunohistochemistry kit |
| Secondary Incubation | 30 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC. |
Fei Long, Zhongnan Hospital of Wuhan University
Verified customer
Submitted 2026-02-12
Product Promise
We promise Anti-FAP1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00422
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 26, 2026