Anti-Hu CD55 Purified Monoclonal Antibody

Product Name

Anti-Hu CD55 Purified Monoclonal Antibody

View all CD55 Antibodies

SKU/Catalog Number

M00910

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD55 Purified Monoclonal Antibody (Catalog# M00910). Tested in Flow Cytometry, IP, IHC-P, WB application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD55 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00910)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-118

Isotype

Mouse IgM

Immunogen

HPB-ALL human T cell line. The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.

Reactive Species

M00910 is reactive to CD55 in Human, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

41.4 kDa

Background of CD55

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00910 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 10 μg/ml.
Immunohistochemistry (paraffin sections): 10 μg/ml.

Validation Images & Assay Conditions

See full target information CD55

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