Product Info Summary
SKU: | M14109 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-RNF39 Mouse Monoclonal Antibody [Clone ID: OTI5E10]
SKU/Catalog Number
M14109
Size
100 µl
Description
Boster Bio RNF39 mouse monoclonal antibody, clone OTI5E10 (formerly 5E10). Catalog# M14109. Tested in IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-RNF39 Mouse Monoclonal Antibody [Clone ID: OTI5E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14109)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5E10
Isotype
IgG1
Immunogen
Full length human recombinant protein of human RNF39 (NP_079512) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M14109 is reactive to RNF39 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
45.525kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14109 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF39.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RNF39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-RNF39 mouse monoclonal antibody (M14109) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF39.
Protein Target Info & Infographic
Gene/Protein Information For RNF39 (Source: Uniprot.org, NCBI)
Gene Name
RNF39
Full Name
RING finger protein 39
Weight
45.525kDa
Alternative Names
HZF; HZFWLTP (long-term potentiation) induced RING finger protein; LIRFHZFw1; Protein HZFw; ring finger protein 39 RNF39 FAP216, HZF, HZFW, LIRF ring finger protein 39 RING finger protein 39|LTP (long-term potentiation) induced RING finger protein|protein HZFw
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF39, check out the RNF39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF39 Mouse Monoclonal Antibody [Clone ID: OTI5E10] (M14109)
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