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- Table of Contents
Facts about Canalicular multispecific organic anion transporter 2.
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Human | |
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Gene Name: | ABCC3 |
Uniprot: | O15438 |
Entrez: | 8714 |
Belongs to: |
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ABC transporter superfamily |
ABC31; ATP-binding cassette sub-family C member 3; ATP-binding cassette, sub-family C (CFTR/MRP), member 3; canalicular multispecific organic anion transporter 2; canicular multispecific organic anion transporter; cMOAT2; EC 3.6.3; EC 3.6.3.44; EC 6.3.2.4; MLP2DKFZp686E22157; MRP3MOAT-DEST90757; multidrug resistance associated protein; Multidrug resistance-associated protein 3; Multi-specific organic anion transporter D
Mass (kDA):
169.343 kDA
Human | |
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Location: | 17q21.33 |
Sequence: | 17; NC_000017.11 (50634881..50692253) |
Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.
Membrane; Multi-pass membrane protein.
When you want to do a specific research on the ABCC3 marker, Boster offers high-affinity primary antibodies for the ABCC3 protein. These antibodies are highly cited and have been validated in multiple applications including Western Blotting, Immunohistochemistry, and ELISA. The research community recommends boster antibody for their specificity and stability.
ABCC3 is a novel marker for progression of EOC. Its overexpression conferred resistance the cytotoxic effects on chemotherapy and other anticancer medications. However, ABCC3 expression is inhibited during colon carcinogenesis, possibly due to Wnt signaling. Therefore, a better understanding of the role of ABCC3 in colon cancer is essential to understand its occurrence.
ABCC3 is a gene that encodes a protein, two mRNAs, and can be expressed in a variety of cancers. The level of ABCC3 affects the therapeutic outcomes of patients with EOC. These genes have been detected using various methods. These methods can currently be used to detect ABCC3 genes in tumors. The highest levels of ABCC3 gene expression are found at SKOV-3 RES. NCI/ADR RES has the lowest levels.
EOC tumors had higher levels of ABCC3 or CPS1 expression than control ovarian tissue. These genes were also increased in resistant ovarian and sensitive ovarian cancer cell line lines. EOC tumors also had higher levels of ABCC3 or CPS1, but this was reversed after taxanes treatment. These markers may be mediators of therapy responses in ovarian tumor cells.
ABCC3 staining results can vary depending upon the methods used. The methods used for staining fixed tissues can vary. DiOlistics is a method that injects DiOlistics ballistically into the neuron. Another method that uses heavy Metals has an indeterminate biological mechanism. While the use of heavy metals is widespread, it may lead to selection bias.
Although there is not much correlation between the different staining methods' differences, the differences can be significant. Both methods target different neuronal populations and operate through different biochemical processes. Different methods can produce different morphology measurements. In one study, the results of Golgi-Cox staining revealed a higher density of dendritic arbors and spine densities compared to those obtained with neurobiotin electroporation.
ABCC3 gene overexpression is associated to acquired MDR in certain cancer cell lines. It is also associated to resistance to 5-fluorouracil for pancreatic and breast cancer cells, as well as doxorubicin. Tetramethylpyrazine reverses overexpression. ABCC3 expression was found to predict NSCLC cancer treatment resistance. ABCC3 inhibition could improve the patient's sensitivity to chemotherapy agents. It may also lead to a new, more targeted therapeutic option.
BenchMark XT automated IHC staining machine was used. The tissues were processed at 4.um and mounted on positively charged slides. After the staining process was completed, tissue slides could be washed in PBS. The slides were then incubated for 15 min in methanol containing 0.3%H2O2. The tissue sections were then incubated with a mouse monoclonal primary antibody against human ABCC3 (1:800 dilution, Abcam).
ABCC3 gene expression is a key marker for studies on the aging process and cardiovascular disease. Boster’s high-affinity prim antibodies are trusted by the research community and are well-cited. These antibodies have been validated for use in Western Blotting, Immunohistochemistry, and ELISA.
PMID: 9813153 by Uchiumi T., et al. Isolation of a novel human canalicular multispecific organic anion transporter, cMOAT2/MRP3, and its expression in cisplatin-resistant cancer cells with decreased ATP-dependent drug transport.
PMID: 9738950 by Kiuchi Y., et al. cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3).