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- Table of Contents
ABCC3 antibody (A02429-1) performs well in ELISA, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP3/ABCC3 Antibody (A02429-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
MRP3/ABCC3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP3/ABCC3 Antibody (A02429-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-MRP3/ABCC3 Antibody Picoband®
ABCC3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC, WB |
Tested Applications
ABCC3 antibody was positively detected by WB in:
human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCC3 antibody was positively detected by IHC in:
human appendiceal adenocarcinoma tissue, human colonic adenocarcinoma tissue, human placenta tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MRP3/ABCC3 Antibody Picoband®
View all ABCC3 Antibodies
View all ABCC3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MRP3/ABCC3 recombinant protein (Position: F214-A275).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
200 kDa
Calculated Molecular Weight
169.3 kDa
Background of ABCC3
Canalicular multispecific organic anion transporter 2 is a protein that in humans is encoded by the ABCC3 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Cite This Product
Anti-MRP3/ABCC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02429-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
74%
Moderate Homology
Dog
73%
Moderate Homology
Cow
71%
Moderate Homology
Sheep
71%
Moderate Homology
Mouse
63%
Low homology
Rat
63%
Low homology
Zebrafish
50%
Low homology
Drosophila
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCC3 antibody
Immunohistochemistry (IHC) protocol for ABCC3 antibody
Publications
Application:
Species:
Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis
Published: 2004-03-14
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.95
Species: Human
PMID: 15149863
Overexpression of ZNRD1 Promotes Multidrug-Resistant Phenotype of Gastric Cancer Cells Through Upregulation of P-Glycoprotein
Published: 2004-02-02
Journal: CANCER BIOLOGY & THERAPY
Application: WB
Impact Factor: 3.02
Species: Human
PMID: 14726695
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Product Promise
We promise Anti-MRP3/ABCC3 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02429-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026