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- Table of Contents
Facts about [Protein ADP-ribosylarginine] hydrolase-like protein 1.
Human | |
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Gene Name: | ADPRHL1 |
Uniprot: | Q8NDY3 |
Entrez: | 113622 |
Belongs to: |
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ADP-ribosylglycohydrolase family |
[Protein ADP-ribosylarginine] hydrolase-like protein 1; ADP-ribosylhydrolase like 1; ADP-ribosyl-hydrolase; ARH2ADP-ribosylhydrolase 2
Mass (kDA):
40.105 kDA
Human | |
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Location: | 13q34 |
Sequence: | 13; NC_000013.11 (113421945..113453488, complement) |
The ADPRHL1 genetic marker is important as it regulates many body processes. There are several markers that can be used for this gene. The most common method for detecting this marker is Western blot. This marker can also be detected using DAB chromogenic detection technology.
Flow Cytometry is a powerful tool for detecting molecules, proteins and cell surface antigens. The samples are either cells or particles. Boster Bio's high-affinity primary antibody can be used to analyze a wide range samples. It is available in monoclonal or polyclonal form. These antibodies are highly cited and continue to be popular in scientific research.
Scientists use the immune system to create specific antibodies when making primary antibodies. These antibodies possess two characteristics: affinity as well as specificity. A high-affinity antibody will bind specifically to an antigen and have low affinity for unintended substances. High-affinity antibodies can detect and purify the targeted antigens. This quality is essential for accurate diagnostics.
Double-label immunofluorescence (DLIF) was used to determine the specific antibody's affinity towards a target protein. This was achieved by incubating the samples using rabbit and goat polyclonal antibody. Both antibodies were diluted to a ratio of 1:100. After incubation, samples were counterstained and dyed using diaminobenzidine and hematoxylin. To prevent detection of an antibody, the negative control was treated with PBS.
ELISA testing is used for antibody development during the development phase. After immunizations, spleen tissue is taken from the animals that have shown the highest levels of response. Hybridoma fusion occurs with the best responders. The limiting-dilution technique subcloning is used to screen positive supernatants. Selected antibodies are then subject to further application testing.
Adprhl1 a ribose bindin protein with a large sequence identity between human Xenopus. This enzyme is found in both the heart tube (and heart tissue). Its activity is required for protein de-ADP-ribosylation. The ribose bindin domain contains tyrosine & serines. These are crucial for stabilizing ADP. D100, the missing residue in hADPRHL1, may be used as an alternative coordination site for metallics by interfering to the phosphate group of forcibly docked ADP.
We used the Invitrogen XCell Surelock Electrophoresis system to analyze lysates of eight human cell lines. Alpha (a)-tubulin protein was detected using a mouse monoclonal antibody, Cat. No. 236-10501 and a goat anti mouse HRP conjugate. Alternatively, we used the XCell Surelock Electrophoresis System.
Human ADPRHL1 does not contain the aprrhl1 gene. Human ADPRHL1 possesses two nucleotide different immediately downstream of ATG. Human ADPRHL1 has a larger region of the 5' sequence, which is required for inhibition. The aprrhl1 gene is not fully conserved, as we discovered. The protein was also expressed in mouse myocardium, in addition to the ADPRHL1 marker.
A study using the FLAG tagged Adprhl1 protein showed that this protein was linked to specific structural modifications in cardiomyocytes. The Adprhl1 hybrid-1-94 protein produced a lot of branched actin filaments. The Adprhl1 natural Xenopus protein was also associated to branched myofibrils.
Surface markers such ADPRHL1 help to determine the chemical's potential to sensitize. They can also help in drug and cosmetic product testing. These markers are expressed by THP-1-cells. These markers can help you determine if a specific product has the potential for sensitizing humans. What are the benefits of this marker? The answer will depend on how you use the marker.
The SITVue/DAB is a powerful Intensification tool that increases the signal strength for chromogenic dyes. It works by depositions two separate Linkers containing horseradish peroxidase. Chromogenic dyes are detected with diaminobenzidine.
There have been many cancers linked to the ADPRHL1 protein, including Alzheimer’s disease. The protein is found in a subset of tumors, which has the highest expression levels. ADPRHL1 may also be found in brain tissue, where it is involved with regulation of cellular functions. Boster Bio has the ADPRHL1 marker. This marker can be used for a variety of applications, including research on cancer.
PMID: 12070318 by Glowacki G., et al. The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse.