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- Table of Contents
Facts about Nucleic acid dioxygenase ALKBH1.
Mainly functions as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed:27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation: modulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed:27745969).
Human | |
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Gene Name: | ALKBH1 |
Uniprot: | Q13686 |
Entrez: | 8846 |
Belongs to: |
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alkB family |
ABH1; ABHalkB, alkylation repair homolog (E. coli); alkB; alkB, alkylation repair homolog 1 (E. coli); ALKBH; alkylated DNA repair protein alkB homolog 1; alkylation repair, alkB homolog; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1; EC 1.14.11.-; EC 4.2.99.18; hABH
Mass (kDA):
43.832 kDA
Human | |
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Location: | 14q24.3 |
Sequence: | 14; NC_000014.9 (77672404..77708038, complement) |
Ubiquitous.
Nucleus. Mitochondrion. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli (By similarity).
Boster Bio's ALKBh2 cytokine is highly specific and can be used for the detection of cancer cells in many samples. Boster Bio has this antibody in stock. We have created a guide to help you understand how to use it. This article will give you information about the Picokine ELISA platform by Boster Bio, including the Picoband and the method for immunohistochemistry/staining.
Picokine(tm), a ELISA kit line, combines a proprietary technology that has been developed over more than 20 year to improve sensitivity. These sensitive ELISA tests can detect a wide range of proteins and antigens. They have a picogram-level sensitivity. Picokine ELISA can detect over 300 biomarkers. To ensure high sensitivity as well as specificity, all the kits have been rigorously evaluated and validated.
Boster Bio's Picokine ELISA systems are suitable for a wide array of applications including cancer research. Picokine ELISA kit are highly sensitive and have been tested in a wide range of applications including IHC and WB. Each kit contains sufficient reagents to perform 96 tests. Boster Bio's Picokine ELISA platform is available through tebu-bio.
Picoband ALKBh2 is an ELISA kit that detects ALKBh2. The Picokine(tm) platform improves sensitivity to the picogram level. It has been validated with a variety samples and is available with images and a validation process. A polymer-based secondary antibody, Supervision, saves time and money in IHC experiments. Sanbio, its distributor, offers technical support and insights into immunogen design for the Picoband.
Immunohistochemistry, a specialized technique that visualizes specific cellular components, is one example. It uses antibodies that recognize and visualize antigens in tissue section. The immunohistochemical products can be visualized using a fluorescent dye, enzyme, radioactive element, or colloidal gold. This allows us to determine the distribution, localization, as well as the cellular contents of cancer-cells.
To do this, vascular sections were removed from the body and rehydrated. Antigen-bound primary antibodies were then used to detect ALKBh2 markers. The endogenous peroxidase activity of the tissues was blocked by incubating the sections for 20 minutes in 0.3% H2O2. The tissue sections were then incubated for 20 minutes in 0.3% H2O2. The staining was done overnight at 4°C using primary antibodies. The stains were visualized using diaminobenzidine. Images were taken with an OLYMPUS microscope BX51.
Knockdown of ALKBh2 reduces aorta calcium mineralization and deposition, and also lowers plasma ALP levels. Two-week-old VSMCs in osteogenic medium with sh–ALKBh2 had decreased calcium deposition and lower ALP activity. ALKBh2 knockdown also decreased the number of calcium nodules in plasma and decreased plasma ALP activity.
Different antibodies react differently to ALKBh2 in different tissues. To ensure consistency, results should be compared. Similarly, the results obtained by two different operators may vary. For instance, different operators might use different washing methods and produce slightly different results. A comparison of sections A and B (Figure 2) is shown below. The staining was performed on the same specimen using the same antibodies.
ALKBh2 regulates BMP2 transcription and promotes BMP2 transcription. ChIP-qPCR is used to determine the effects of ALKBh2 knockout upon BMP2 levels. ALKBh2 knockout reduces BMP2 expression in HASMCs.
Oct4 regulates ALKBh2's expression in the VSMC, thereby mediating its procalcifying effect. ALKBh2 deficiency in nephrophrectomy-induced mice inhibited osteogenic reprogramming. VSMCs that have ALKBh2 elevated become osteogenic. It also promotes osteogenic programmed reprogramming during calciumification.
PMID: 8600462 by Wei Y.F., et al. Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair.
PMID: 17979886 by Tsujikawa K., et al. Expression and sub-cellular localization of human ABH family molecules.