Apolipoprotein A-V Antibodies

Apolipoprotein A-V (Apoa5)

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons.

Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production speed (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages (By similarity).

Gene Information

Mouse
Gene Name:	Apoa5
Uniprot:	Q8C7G5
Entrez:	66113

Family

Belongs to:
apolipoprotein A1/A4/E family

Alternative Names

APOAV; apo-AV; APOA-V; Apolipoprotein A5; apolipoprotein A-V; FLJ97995; MGC126836; RAP3MGC126838; Regeneration-associated protein 3

Molecular Weight

Mass (kDA):
41.262 kDA

Genomic Context

Mouse
Location:	9|9 A5.2
Sequence:	9;

Tissue Specificity

Liver.

Diseases

• Cardiovascular Diseases
• Diabetes Mellitus
• Obesity
• Coronary Heart Disease
• Coronary Artery Disease
• Heart Diseases
• Hyperlipidemia
• Dyslipidemias
• Diabetes Mellitus, Non-insulin-dependent
• Cholangiocarcinoma
• Metabolic Syndrome X
• Atherosclerosis
• Dysequilibrium Syndrome

• Infarction
• Hypertensive Disease
• Myocardial Infarction
• Cerebrovascular Accident
• Combined Hyperlipidaemia
• Liver Carcinoma
• Insulin Resistance

Pathways

• Secretion
• Pathogenesis
• Lipid Homeostasis
• Triglyceride Homeostasis
• Response To Fenofibrate
• Transport
• Endocytosis
• Glucose Homeostasis
• Lipid Binding
• Liver Regeneration
• Lipid Transport
• Regeneration
• Aging

• Translation
• Cholesterol Efflux
• Menopause
• Gene Silencing
• Proteolysis
• Histone Modification
• Sexual Reproduction

PTMs

• Oxidation
• Phosphorylation

• Dephosphorylation
• Cleavage

• Acetylation

Research Areas

• Cancer
• Cholesterol Metabolism
• Lipid and Metabolism
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Apoa5

Best Uses Of The APOA5 Marker From Boster Bio

If you're looking to learn more about the APOA5 marker you've found the right website. Learn more about the APOA5 monoclonal antibody and the gene APOA5. In this article, we'll go over the most commonly used uses for the APOA5 APOA5 gene and marker. If you're a scientist seeking to publish results from study of species or their applications You'll discover that a Boster Bio product is an excellent choice.

APOA5 Marker

Monitoring the levels of triglycerides and determining the impact of genetic changes in metabolic lipids are two the most beneficial uses of the APOA5 marker. Although the marker is most frequently found on HDL and VLDL particles, it is also able to be found on HDL particles. Although its impact on the levels of lipids is not significant but the presence of variants in plasma may indicate possible pathologies. While there isn't much evidence to support a direct effect of APOA5 in reducing LDLc, it is worth investigating its role in hyperlipidemia.

Three polymorphic alleles form the APOA5 gene. These alleles are called haplotypes. The most frequent of these alleles defines the APOA5 haplotypes. The -1131C allele defines the APOA5*2 haplotype. the c.56G allele defines APOA5*3. Both minor alleles were found among high-risk populations like blacks and Hispanics. This supports the idea of the polymorphisms being a result of evolution earlier in the history of humanity.

Researchers discovered that minor APOA5 variants have been linked with high triglyceride as well as lower levels of triglycerides. While the function of ApoA5 in the levels of triglycerides has not been determined, it appears to be involved in other processes such as the size and length of LDL particles as well as the levels of triglycerides. ApoA5 variants have also been linked to other risk factors in the cardiovascular system.

In Asians, the APOA5 variant c.56C-G is associated with an increased risk of MetS as well as a distinct relationship was observed in Europeans. However, more studies are needed to confirm these associations in larger populations to determine the significance of the APOA5 variant in MetS. Therefore, it is crucial to determine if APOA5 variants are associated with HDL levels to better understand the risk of MetS.

The APOA5 gene is found on the human chromosome 11 in the position 11q23. It contains four exons, a paralogous gene relation, and is about 366 amino acids. The gene is predominantly expressed in the liver, however it has also been found to be expressed in the small intestine. The gene is involved in liver regeneration. Because of this, researchers are currently investigating its role in the process of liver regeneration.

Monoclonal antibody APO5

Boster Bio has the monoclonal anti-Apolipoprotein V APOA5 monoclonal antibody under the catalog number A01242-1. The antibody reacts with ApoA5 in human serum. The product was immunoprecipitated using an anti-ApoA5 antibody , as well as the ANGPTL3/8 complex. The results showed that both peptides are co-immunoprecipitated by the antibody.

The company provides high-quality monoclonal antibodies to research. Their quality control and research support services assure you of the right product every time. Boster Bio is a trusted supplier of high-quality antibodies and their friendly customer service will be of great help. Visit the Boster Bio website for more information. It also offers custom services as well as BeNeLux delivery services. We are a trusted supplier for APOA5 research and development.

This test was performed using LPL-stable human expression cells that were seeded in poly D-lysine-coated plates with 96 wells. The cells were incubated overnight and then replaced with 80 ml medium which contained ANGPTL complexes in serially diluted form or proteins. For one hour, incubation was done at 37°C. A Titer-sensitive method was utilized to determine the ApoA5 antibody's affinity for ANGPTL3/8.

LPL activity tests were performed with a 4-parameter nonlinear logistic regression modeling. The fluorescence was measured every minute and the numbers were plotted against time to show levels of inhibition of LPL. The curves of calibration for immunoassays were constructed using MSD software. ApoA5 immunoassays employed a 5-parameter fit and weighting using 1/y2. The resulting log2 mean areas under the curves was compared by using the Student's test. Before statistical analysis, the extract AUC was reduced to a log2 of 10.

Informationgraphics on APOA5 genes

The APOA5 gene encodes a protein that is important in regulating plasma triglyceride levels, which are a risk factor for coronary artery disease. Apolipoprotein AV is similar to the rat's protein and increases in response to liver injury. The gene has been linked to hypertriglyceridemia, a metabolic disorder marked by high triglyceride levels. The APOA5 gene can be located on chromosome 11, close to the apolipoprotein cluster.

Numerous studies have looked into the relationship between common APOA5 variants and heart disease risk. These interactions involve dietary habits (eating of polyunsaturated fat acids) as well as exercise and consumption of alcohol. Although there is no definitive evidence of a causal link between the APOA5 gene variants and risk of cardiovascular disease, studies have shown that SNPs that are associated with risk for obesity are present in two different regions of the genome.

APOA5 gene infographics from Boster Bio feature basic information about the APOA5 gene. These infographics cover all genes found in mouse and human. They also contain a search bar that allows you to find a specific gene. The APOA5 gene information in a variety of ways and find out which one works best for you. After you've discovered more about the gene, it's time to take action.

The APOA5 gene is closely related to obesity and metabolic syndrome. Numerous studies have proven an connection between APOA5 gene variants and a higher risk of metabolic syndrome and obesity. Genetic studies haven't revealed a causal relationship between APOA5 variants and cardiovascular disease. This suggests that the gene may be context-dependent. If this is so, more research is needed to prove the connection.

The study of APOA5 and APOC3 polymorphisms revealed that S19W and -1131T,C were both associated with higher plasma triglyceride levels. However, this finding did not correspond with other races or groups. The baseline data were used to help the authors to make their conclusions. The study's findings may not be applicable to other populations or races since the diets of participants can alter based on their environment.

APOA5 gene

The APOA5 gene is involved in the production of a variety of proteins within the body. With this gene, scientists can identify its expression in different tissues. The gene is present in a variety of tissues, including the liver, intestines and spleen. Therefore, identifying the protein through the APOA5 marker is important for research purposes. However, the most effective application of this gene isn't yet understood.