Neutral ceramidase Antibodies

AND Asah2 ELISA kits, Neutral ceramidase Proteins

Many biological assays use antibodies to detect Neutral ceramidase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Neutral ceramidase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Neutral ceramidase antibodies...

We validate the specificity of these antibodies to Neutral ceramidase by testing them on tissues known to express Asah2 positively and negatively. Browse below to find the Asah2 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: Septin9 Next: Cpsf3

Asah2 Infographic by Boster Bio

All Asah2 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Neutral ceramidase ASAH2 Antibody

Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL

Human, Mouse, Rat

ELISA, WB

$481
Cat # A04725
0.1 mg

Anti-Neutral ceramidase ASAH2 Antibody

Western blot analysis of extracts of various cell lines, using ASAH2 Rabbit pAb at 1:500 dilution

Human, Mouse, Rat

$405
Cat # A04725-1
100ul

Human ASAH2 ELISA Kit PicoKine®

Human

ELISA

$750
Cat # EK2134
96 wells/kit, with removable strips.

ASAH2 (NM_019893) Human Over-expression Lysate

Human

$960
Cat # LS056624
20 µg, 100 µg

Asah2 Overview

Facts about Neutral ceramidase.

Neutral ceramidase (Asah2)

Hydrolyzes the sphingolipid ceramide to sphingosine and free fatty acid at an optimum pH of 6.5-8.

5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine in the cell surface.

Gene Information

Mouse
Gene Name:	Asah2
Uniprot:	Q9JHE3
Entrez:	54447

Family

Belongs to:

neutral ceramidase family

Alternative Names

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase

Molecular Weight

Mass (kDA):
83.509 kDA

Genomic Context


Mouse

Location:	19\|19 C1
Sequence:	19;

Tissue Specificity

Widely expressed. Strongly expressed in liver and kidney. Highly expressed in the small intestine along the brush border. Localizes in the apical membranes of proximal and distal tubules, collecting ducts of kidney, endosome-like organelles of hepatocytes, and in the epithelia of the jejunum and ileum.

Diseases

Diabetic Nephropathy
Kidney Diseases
Enterocolitis, Necrotizing
Retinal Degeneration
Neurodegenerative Disorders
Chronic Hyperglycemia
Dermatitis, Atopic
Malignant Tumor Of Colon
Enterocolitis
Ulcer
Carcinoma, Neuroendocrine
Hemolysis (disorder)

Lysosomal Storage Diseases
Malignant Neoplasms
Hyperglycemia
Chimera Disorder
Colitis
Infective Disorder
Abnormal Degeneration

Pathways

Cell Proliferation
Localization
Transport
Pathogenesis
Translation
Mrna Transcription
Cell Death
Lipoprotein Transport

Cell Differentiation
Phototransduction
Virulence

PTMs

Phosphorylation
Glycosylation
Acylation

Cleavage
Oxidation
Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Asah2

References

PMID: 10753931 by Tani M., et al. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases.

PMID: 10652340 by Tani M., et al. Purification and characterization of a neutral ceramidase from mouse liver. A single protein catalyzes the reversible reaction in which ceramide is both hydrolyzed and synthesized.