Neutral ceramidase Antibodies

Neutral ceramidase (Asah2)

Hydrolyzes the sphingolipid ceramide to sphingosine and free fatty acid at an optimum pH of 6.5-8.

5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine in the cell surface.

Gene Information

Mouse
Gene Name:	Asah2
Uniprot:	Q9JHE3
Entrez:	54447

Family

Belongs to:
neutral ceramidase family

Alternative Names

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase

Molecular Weight

Mass (kDA):
83.509 kDA

Genomic Context

Mouse
Location:	19|19 C1
Sequence:	19;

Tissue Specificity

Widely expressed. Strongly expressed in liver and kidney. Highly expressed in the small intestine along the brush border. Localizes in the apical membranes of proximal and distal tubules, collecting ducts of kidney, endosome-like organelles of hepatocytes, and in the epithelia of the jejunum and ileum.

Diseases

• Diabetic Nephropathy
• Kidney Diseases
• Enterocolitis, Necrotizing
• Retinal Degeneration
• Neurodegenerative Disorders
• Chronic Hyperglycemia
• Dermatitis, Atopic
• Malignant Tumor Of Colon
• Enterocolitis
• Ulcer
• Carcinoma, Neuroendocrine
• Hemolysis (disorder)

• Lysosomal Storage Diseases
• Malignant Neoplasms
• Hyperglycemia
• Chimera Disorder
• Colitis
• Infective Disorder
• Abnormal Degeneration

Pathways

• Cell Proliferation
• Localization
• Transport
• Pathogenesis
• Translation
• Mrna Transcription
• Cell Death
• Lipoprotein Transport

• Cell Differentiation
• Phototransduction
• Virulence

PTMs

• Phosphorylation
• Glycosylation
• Acylation

• Cleavage
• Oxidation
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Asah2

Best Uses Of The ASAH2 Marker From Boster Bio

Boster offers high-affinity primary antibodies to the ASAH2 Marker. Boster has been a trusted name in research for over 25 years. The antibodies used for this research are well-validated on Western Blotting, Immunohistochemistry, and ELISA. The only one that targets ASAH2 is boster antibody.

Primary antibodies of high-affinity

Boster Bio's product range is a great choice if you are looking for high-quality primary antibodies. They specialize in high-specificity and high-sensitivity antibodies, and have developed over 12,000 of them, resulting in antibodies that are both sensitive and specific. These antibodies are quantitatively validated against a panel consisting of 250 tissues, untransfected line cells, and other recombinant proteins.

The company's high-affinity, primary antibodies have earned it a strong market reputation. The company produces antibodies for gene transcription analysis, DNA andRNA purification, protein extraction, purification, and gene expression analysis. Bioworld was founded in 1993 and is one of the leading suppliers of phosphophospho-antibodies. With more than 500 phospho-antibodies under its belt, Boster Bio is a market leader in this area.

Primary antibodies are derived from the immune systems of the host. They react with antigens. These proteins are composed from regions called complementarity-determining regions. This Wikipedia article provides information on these regions. Scientists make polyclonal prima antibodies by immunizing host animal with antigens and then extracting recombinant proteins form the sera or eggs.

According to the OAS hypothesis, new influenza virus strains can escape surveillance if the immune system is largely absent. The new variant will be able to evade surveillance because the immune system is too dependent on memory B cells. A recent study conducted by researchers at the University of Texas in Austin, TX, found that anti-B/Malaysia/2506/2004 antibodies bind to both B and C strains at the same affinity.

Primary antibodies are highly specific because they bind to the target antibody in an aqueous medium. This property is important in experiments that use the antibodies for specific purposes. A good antibody can detect the antigen, purify it, and measure its level in the body. What are primary antibodies? What are primary antibodies and what are their uses? If they are used in a laboratory, they can be used for research, diagnosis, and other diagnostic purposes.

The principle of enzyme -linked immunosorbent tests is similar to other immunoassay methods. They use specific antibodies to bind the target antigen and a detection system for confirmation. Boster Bio high affinity primary antibodies are able to increase the sensitivity for assays. Scientists from all over the world have come relying on the high affinity primary antibodies manufactured by Boster Bio.

Gene infographics

To create gene infographics based off the ASAH2, the genetic code for ASAH2 is required. This gene is located in the mouse genome. You can also find information about other species. ASAH2 can be used to prevent cancer and other important functions. There are two versions of the gene: the human and mouse. The mouse form is expressed in the liver.

Histories

Human ASAH2 was initially discovered in the small intestinal tract. It is now found on human embryonic and adult kidney cells. Hwang and coworkers discovered that ASAH2 in human embryonic kidney cells is highly glycosylated and O-glycans bound. It is also found at the plasma membrane. Tani and coworkers found that ASAH2 was retained in the ER, which is where it displays activity. Sakamoto, coworkers, and others identified the ASAH2 protein in human colon cancer cells. They found that it was largely located in Golgi which is the storage compartment of a cell.

ASAH2 regulates basal activation for AKT in cells. This makes it a potential target for treatment of colon cancer. But, more research is needed in order to find the right animal model. The brain function after TBI was partially improved by ASAH2 gene knockdown in mice. This suggests that ASAH2 might be involved in traumatic head injury. The next step in this process is to determine if there's a direct link between ASAH2 traumatic brain Injury (TBI).

ASAH2 is responsible for the production and function of neutral ceramidase. This enzyme is vital for the proper digestion of sphingolipids. ASAH2 regulates the levels ceramide, sphingosine and other intestinal enzymes. This could have important effects on the biology and development of the intestinal microbiome and the disease process. However, very little is known about its role in the human body.

ASAH2 inhibitors are necessary for treatment of colorectal tumors. Inhibition of the protein increases ceramide levels, inhibits ERK signaling, and induces apoptosis. This drug has been approved in Japan for the first time and is an effective treatment of colon cancer patients. Recent research demonstrated that ASAH2 inhibited tumor development in a xenograft-based model.