ASGR1 Antibodies

Asialoglycoprotein receptor 1 (ASGR1)

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue in their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units.

After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Gene Information

Human
Gene Name:	ASGR1
Uniprot:	P07306
Entrez:	432

Family

Belongs to:
No superfamily

Alternative Names

ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1

Molecular Weight

Mass (kDA):
33.186 kDA

Genomic Context

Human
Location:	17p13.1
Sequence:	17; NC_000017.11 (7173431..7179564, complement)

Tissue Specificity

Expressed exclusively in hepatic parenchymal cells.

Subcellular Localizations

[Isoform H1a]: Membrane; Single-pass type II membrane protein.; [Isoform H1b]: Secreted.

Diseases

• Hepatitis
• Tissue Adhesions
• Keratosis
• Liver Diseases
• Neoplasm Metastasis
• Dizziness
• Colonic Neoplasms
• Atrichia
• Malignant Tumor Of Colon
• Hepatitis B
• Cyst, Intracranial Arachnoid
• Carcinoma
• Fibrosis

• Neoplasms
• Headache
• Hyperglycemia
• Viral Hepatitis
• Colorectal Cancer
• Retinal Diseases
• Bone Diseases

Interacting Proteins

• CERS2

Pathways

• Phagocytosis
• Transport
• Reverse Transcription
• Keratinization
• Cell Proliferation
• Translation
• Mating
• Pathogenesis

• Conjugation
• Bone Mineralization

PTMs

• Disulfide bond
• Glycoprotein

• Lipoprotein
• Palmitate

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ASGR1

Boster Bio Anti-Asialoglycoprotein Receptor 1 ASGR1 Antibody

Anti-Asialoglycoprotein receptor 1 ASGR1 Antibody is available from Boster Bio with catalog number A05376. This monoclonal antibody reacts with Rat, Mouse, and Human proteins. Its use in immunohistochemistry can be found below. There are many uses of ASGR1 Marker. Here are some of the best. You can use Boster Bio Anti-Asialoglycoprotein receptor 1 ASGR1 Antibody in your research.

Anti-Asialoglycoprotein receptor 1 ASGR1 Marker

Asialoglycoprotein-receptor 1 ASGR1 a C-type of lectin is expressed on hepatocytes' sinusoidal surface. Anal Morell and Gilbert Ashwell discovered the receptor in 1965. They isolated the protein from rabbit liver using affinity Chromatography and asialosomucoid Sepharose.

Asialoglycoprotein receptor 1 is a transmembrane glycoprotein receptor that mediates the endocytosis and lysis of glycoproteins in the liver. This protein could facilitate hepatic infection by multiple viral pathogens, such as hepatitis B. ASGR1 is a potential target for liver specific drug delivery. The antibody has been tested on Rat, Mouse and Human tissues.

Applications

ASGR1 is a ligand binding glycoprotein. It binds a terminal sialic acid, as its name suggests. Loss of the terminal sialic acid leads to rapid internalization of asialoglycoproteins by liver cells. As the ligand binds a receptor, a dissociation of the two cations occurs. The ligand flows through the vacuole into the lysosome to produce degradation products. 60 minutes after the initial binding of a receptor, the ligand is dissociated. Acidification of the endosome is inhibited by weak bases after dissociation.

The ASGR1 protein is expressed on human monocytes. The ASGR2 genome encodes two forms, the membrane-bound form and the soluble form. ASGR2 expression is ininversely correlated to monocyte gene expression. ASGR1 is found in human macrophages and mouse testis. However, it is low in these tissues.

ASGR1 encodes a transmembrane-protein that plays a crucial role in the endocytosis desialylated blood proteins. ASGR1 expression is high at many critical stages of human growth. It is seventy percent more abundant in totipotent 8-cell embryogenesis that in later embryonic and stem cells. During differentiation, ASGR1 expression increases threefold in mouse multipotent stem cell lines.

RTPCR was used in order to detect ASGR1 transcripts T1 &T2 in the fraction of M and L cells. The RT-PCR products are highly reproducible and were derived RNA taken from 6 individuals. The results were analysed by polyacrylamide gel Electrophoresis. The distribution of transcripts can be seen in the figure (Figure).

ASGR1-expressing HLCs display the same functional characteristics and gene expression profile as primary hepatocytes. ASGR1+ HLCs can be used in functional analysis. ASGR1+ cells have higher expression of hepatocyte marker genetics than unsorted HLCs and inefficient differentiations. This makes ASGR1+ HLCs a great candidate for sorting human sperm cells.

Biotinylated Human ASGR1 is compatible with mo-DCs. The ASGR1-H5253 antibody is compatible with mo-DCs. It takes approximately 5 min to internalize when it is used with mo DCs. The error bars are standard errors of the mean. However, dilutions must be optimized by each laboratory.

Synonyms

ASGR1 is a hepatic membrane protein that has been used over many years to detect circulating hepatocellular carcinoma cell lines and to purify hPSC derived hepatocytes. The ASGR1 protein encodes 291 amino acid residues that function in carbohydrate binding as well as post-translational modification of protein. It is a highly expressed protein that can be found on most human cells.

As a member of the family of C-type lectins, ASGR1 recognizes a diverse set of ligands, including terminal galactose and N-acetylgalactosamine. It is found mainly in human liver cells. It is composed of two noncovalently connected subunits, the major ASGR1 proteins and the minor ASGR1 glyprotein.