This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
BMPR1A / Bone morphogenetic protein receptor type-1A · Western blot design guide
Design a Western Blot for BMPR1A
Real validated BMPR1A Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-BMPR1A WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
BMPR1A Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~60.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Cerebral cortex (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Cleaved | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated BMPR1A Western Blot Protocols
The A01581 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysates from 293, mouse NIH/3T3 cell line (from left to right), (catalog A01581) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01581; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | goat anti-rabbit IgG, 1:5000 (catalog A01581) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected BMPR1A Western Blot Band Size?
BMPR1A has a predicted 60.2 kDa precursor; signal-peptide cleavage and Asn73 glycosylation may affect migration, but no empirical band size or shift is established.
| Band near 60.2 kDa | consistent with the predicted full-length BMPR1A precursor; confirm identity with controls |
| Band slightly below the precursor position | could reflect removal of the 1–23 signal peptide |
| Band above the predicted position | N-linked glycosylation at Asn73 could affect migration, but its extent is unknown |
| Weak band in a soluble lysate fraction | cell-membrane BMPR1A may be underrepresented |
💡Expected BMPR1A appearanceUniProt predicts a 60.2 kDa full-length precursor; signal-peptide removal and N-linked glycosylation at Asn73 may affect migration, but no empirical band size is supplied, so confirm any candidate band with identity controls.
| Predicted full-length mass | places the 532-residue precursor at 60.2 kDa |
| Full-length precursor sequence | includes the signal peptide in the 60.2 kDa prediction |
| Signal peptide at residues 1–23 | its removal could make the mature receptor smaller than the precursor |
| N-linked glycosylation at Asn73 | could change apparent migration; the size of any effect is unestablished |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | membrane-localized BMPR1A may be poorly recovered | check membrane extraction and compare with a membrane-enriched fraction |
| Band higher than expected | N-linked glycosylation at Asn73 could affect migration, but band identity is uncertain | compare glycosidase-treated and untreated samples and verify identity with BMPR1A depletion |
| Band lower than expected | signal-peptide removal may reduce size | check antibody epitope coverage and verify the band with BMPR1A depletion |
| Multiple bands | their identities are unresolved; the listed features do not establish distinct bands | use BMPR1A depletion and compare glycosidase-treated samples |
| Weak or no signal | cell-membrane BMPR1A may be underrepresented in the prepared sample | check membrane-protein recovery and include a positive-control lysate |
Experiment evidence
Western blot images showing BMPR1A band
A01581 · BMPR1A
A01581 · BMPR1A
A01581-1 · BMPR1A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for BMPR1A Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for BMPR1A in Western blot, you can use cerebral cortex tissue, where HPA reports high expression.
Positive control: Cerebral cortex (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: As a cell-membrane protein, BMPR1A may be easier to detect in membrane-enriched lysates.
HPA tissue expression evidence for BMPR1A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebral cortex | endothelial cells | High | Protein (IHC) | HPA → |
| Colon | endothelial cells | High | Protein (IHC) | HPA → |
| Gallbladder | glandular cells | High | Protein (IHC) | HPA → |
| Pancreas | exocrine glandular cells | High | Protein (IHC) | HPA → |
| Placenta | trophoblastic cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Fallopian tube | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced BMPR1A Western Blot Tips
Deeper troubleshooting and optimisation questions for BMPR1A, answered from its protein features.
How should BMPR1A band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could BMPR1A isoforms explain multiple bands?
Isoforms · The supplied features list one isoform and no alternative sequence. They provide no basis for assigning multiple bands to distinct BMPR1A isoforms.
Is BMPR1A glycosylated?
PTM · The supplied features list one N-linked glycosylation site, Asn73 in UniProt numbering. Compare treated and untreated samples if assessing its contribution to an observed band; the annotation alone does not predict the size of a shift.
Does this guide establish induction of BMPR1A?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for BMPR1A?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01581 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should BMPR1A bands be quantified across samples?
Quantitation · Because BMPR1A is a membrane protein, prepare samples consistently and compare equivalent loaded material, including membrane-containing material. The supplied features provide no induction condition or expected change in abundance.
Why might a BMPR1A band differ from 60.2 kDa?
Interpretation · 60.2 kDa is the predicted mass, not an observed band position. BMPR1A has a signal peptide at residues 1–23 and an N-linked glycosylation site at Asn73, using the supplied UniProt numbering. These features may affect the protein's mass, but they do not establish a visible shift or explain a particular band difference.
BMPR1A is annotated at the cell membrane and cell surface as a single-pass type I membrane protein. Retain the membrane-containing material during sample preparation so it is represented in the material loaded for the blot.
Five disulfide bonds are annotated. Keep reducing conditions consistent across samples when comparing bands. The supplied features do not establish whether changing those conditions would alter BMPR1A's apparent position.
Check its position against the predicted 60.2 kDa mass, then consider the annotated signal peptide at residues 1–23 and N-linked site at Asn73. These UniProt features suggest possibilities to investigate; they do not identify an unexpected band or establish its cause.
BMPR1A is annotated at the cell membrane and cell surface as a single-pass type I membrane protein. Retain the membrane-containing material during sample preparation so it is represented in the material loaded for the blot.
Five disulfide bonds are annotated. Keep reducing conditions consistent across samples when comparing bands. The supplied features do not establish whether changing those conditions would alter BMPR1A's apparent position.
Check its position against the predicted 60.2 kDa mass, then consider the annotated signal peptide at residues 1–23 and N-linked site at Asn73. These UniProt features suggest possibilities to investigate; they do not identify an unexpected band or establish its cause.
Boster reagents
BMPR1A Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-BMPR1A Antibody (C-term)
Cat # A01581
Real WB data
Anti-BMPR1A Antibody Picoband®
Cat # A01581-1
Both listed anti-BMPR1A antibodies have Western blot images in the reported samples. A01581 was shown with 293 and mouse NIH/3T3 lysates; A01581-1 was shown with human 293T and MCF-7 cells and rat skeletal muscle. The supplied evidence does not show independent validation.
Which to pick: Choose A01581 for mouse NIH/3T3 lysates or A01581-1 for rat skeletal muscle lysates. For human cells, compare the reported samples: 293 for A01581, or 293T and MCF-7 for A01581-1. Both have WB images.
Source: BosterBio BMPR1A gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P36894.
- Human Protein Atlas. BMPR1A tissue expression.
- PMC11116843 — target-verified WB comparison
- PMC13315359 — target-verified WB comparison
- PMC4344833 — target-verified WB comparison