BRD3 Antibodies

AND BRD3 ELISA kits, BRD3 Proteins

Many biological assays use antibodies to detect BRD3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BRD3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BRD3 antibodies...

We validate the specificity of these antibodies to BRD3 by testing them on tissues known to express BRD3 positively and negatively. Browse below to find the BRD3 antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for BRD3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the BRD3 Western Blot Guide

BRD3 Infographic by Boster Bio

All BRD3 Products

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Product

Figures

Specificity

Applications

Price

Anti-BRD3 Antibody Picoband®

Western blot analysis of BRD3 using anti-BRD3 antibody (A04044-3)

Flow Cytometry analysis of 293T cells using anti-BRD3 antibody (A04044-3)

Human

Flow Cytometry, WB

$370
Cat # A04044-3
100 μg/vial

Anti-BRD3 Antibody

Western blot analysis of extracts of various cell lines, using BRD3 antibody at 1:1000 dilution

Immunohistochemistry of paraffin-embedded rat testis using BRD3 antibody at dilution of 1:100

Human, Rat

IF, IHC, ICC, WB

$405
Cat # A04044-2
100ul

Anti-BRD3 (F645) Antibody

Western blot (WB) analysis of BRD3 pAb at 1:1000 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:Jurkat whole cell lysate(40ug)

Human

IHC, WB

$405
Cat # A04044-1
100ul

Anti-BRD3 Antibody

Western Blot analysis of various cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit

Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit

Immunohistochemical analysis of paraffin-embedded human Colon cancer

Human, Mouse

ELISA, IF, IHC, WB

$399
Cat # A04044
100μl

BRD3 Colorimetric Cell-Based ELISA

Western blot analysis of extracts from A549 cells

Human, Mouse

ELISA

$480
Cat # EKC1816
1 kit, containing one 96-well plate and all necessary reagents

BRD3 (NM_007371) Human Over-expression Lysate

Human

$960
Cat # LS008019
20 µg, 100 µg

Human BRD3 Recombinant Protein

SDS-PAGE

$250
Cat # PROTQ15059
Starting from 20ug

BRD3 Overview

Facts about Bromodomain-containing protein 3.

BRD3 3D structure. Source: alphafold

Bromodomain-containing protein 3 (BRD3)

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114). Regulates transcription by promoting the binding of the transcription factor GATA1 to its aims (By similarity).

Gene Information

Human
Gene Name:	BRD3
Uniprot:	Q15059
Entrez:	8019

Family

Belongs to:

No superfamily

Alternative Names

bromodomain containing 3; bromodomain-containing protein 3; KIAA0043FLJ41328; ORFXbromodomain-containing 3; RING3LFLJ23227; RING3-like protein

Molecular Weight

Mass (kDA):
79.542 kDA

Genomic Context


Human

Location:	9q34.2
Sequence:	9; NC_000009.12 (134030305..134068533, complement)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Nucleus. Detected on chromatin.

Diseases

Carcinoma
Neoplasms
Malignant Neoplasms
Cancer Of Nasopharynx
Neoplasm Of Uncertain Or Unknown Behavior Of Mediastinum
Neoplasm Metastasis
Carcinogenesis
Pancreatic Neoplasm
Azoospermia
Nasopharyngeal Neoplasms
Chromosomal Translocation
Cystic Fibrosis
Local Disease

Epithelioma
Metastatic Malignant Neoplasm To The Lung
Thymus Neoplasms
Congenital Bilateral Aplasia Of Vas Deferens
Thymoma
Congenital Bilateral Absence
Infertility

Interacting Proteins

Pathways

Cell Cycle
Localization
Cell Cycle Arrest
Pathogenesis
Spermatogenesis
Histone Acetylation
Cell Proliferation
Diplotene
Interaction With Host
Mitosis
Histone Modification
Sex Determination
Positive Regulation Of Cell Proliferation

Methylation
Dna Replication
Establishment Of Viral Latency
Viral Latency
Regulation Of Cell Proliferation
S Phase
Cell Growth

PTMs

Acetylation

Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BRD3

References

PMID: 16008511 by Ishii H., et al. Differentially expressed genes in endothelial differentiation.

PMID: 9373153 by Thorpe K.L., et al. Chromosomal localization, gene structure and transcription pattern of the ORFX gene, a homologue of the MHC-linked RING3 gene.