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Facts about Voltage-dependent L-type calcium channel subunit alpha-1D.
Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines.
Human | |
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Gene Name: | CACNA1D |
Uniprot: | Q01668 |
Entrez: | 776 |
Belongs to: |
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calcium channel alpha-1 subunit (TC 1.A.1.11) family |
CACN4calcium channel, neuroendocrine/brain-type, alpha 1 subunit; CACNL1A2voltage-dependent L-type calcium channel subunit alpha-1D; Calcium channel, L type, alpha-1 polypeptide, isoform 2; calcium channel, voltage-dependent, L type, alpha 1D subunit; Cav1.3; CCHL1A2voltage-gated calcium channel alpha 1 subunit; L type, alpha-1 polypeptide; voltage-gated calcium channel alpha subunit Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3
Mass (kDA):
245.141 kDA
Human | |
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Location: | 3p21.1 |
Sequence: | 3; NC_000003.12 (53494611..53813733) |
Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
Membrane; Multi-pass membrane protein.
This guide provides Boster Bio optimization tips that will help you improve your experiments. This guide will aid you in improving the performance of your experiments and produce better results. Every researcher is bound to encounter problems during their research. Although proper controls can eliminate the majority of sources of error, there are still some problems that may arise. Boster Bio includes troubleshooting guides that can help you identify possible problems and figure out the most effective solution.
If you're a scientist looking to maximize your results using the CACNA1D marker you've come to the right spot. Boster offers a variety of methods to maximize your results including the preparation of your sample and the protocol. These suggestions are applicable to all scientists around the globe:
You're in the right place if are looking for an immunohistochemistry tutorial for the CACNA1D marker. Boster Bio has compiled a range of resources to help you with your tests. The guides contain information on the basics of immunohistochemistry, including methods for strong and weak staining, how to perform optimization techniques, and more. They're an invaluable resource, and offer a comprehensive overview of the most frequent IHC problems and tips.
To design the custom zebrafish/c. elegans genome-edited models for CACNA1D We began by identifying a potential gene. Next, we created cDNA libraries using the Superscript III reagent from the crude and purified total RNA. Next, we used the Platinum Taq DNA Polymerase to amplify the cDNA libraries using primer sets that are specific to the gene.
The STXBP1 human code sequence was employed. It was inserted between the C. the elegans ortholog gene and the homology arms downstream of the human gene. This resulted in an allele that was deleted from the ortholog, which can be verified using a tagged epitope. To create a null ortholog, we constructed another repair template.
MOs are synthetic Oligonucleotides which inhibit translation and splicing of mRNAs. MOs can be used to decrease expression of genes in F0 animals. At 4dpf, MO levels have fallen to sub-optimal levels. Knockdown is also suboptimal.
Human homologous sequences are essential to produce accurate and reproducible genome-edited CACNA1D models. This isn't without issues. A full-length DAF-18 model isn't likely to be accurate due its poor homology-based model of the unconserved C-terminus region. We use the InVivo Biosystems Custom Zebrafish/c. To optimize drug discovery we employ genome-editing models of elegans to study CACNA1D.
The resulting genome-edited zebrafish/c. is human-derived. The CACNA1D genome-edited models of zebrafish/c created from elegans are extremely relevant and reproducible. There are numerous advantages to the human gene-replacement approach. Human-derived ortholog deletion alleles can be produced in any species, removing confounding effects of background mutations. The excisable selectable markers don't change the morphology of the animal, baseline locomotion as well as a range of evoked sensorimotor behaviors.
InVivo Biosystems Custom zebreafish/c. Genomic technology has been employed to create genome-edited models CACNA1D in elegans. These models offer important insights for drug discovery. In addition the custom zebrafish/c. The CACNA1D genome-edited models of elegans have been successfully translated into a variety of human diseases which includes CACNA1D.
PMID: 1309651 by Williams M.E., et al. Structure and functional expression of alpha 1, alpha 2, and beta subunits of a novel human neuronal calcium channel subtype.
PMID: 1309948 by Seino S., et al. Cloning of the alpha 1 subunit of a voltage-dependent calcium channel expressed in pancreatic beta cells.
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