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Product Info Summary
| SKU: | PA2293 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CaV1.3/CACNA1D Antibody Picoband®
SKU/Catalog Number
PA2293
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CaV1.3/CACNA1D Antibody catalog # PA2293. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaV1.3/CACNA1D Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2293)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CaV1.3, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2293 is reactive to CACNA1D in Human, Mouse, Rat
Observed Molecular Weight
245 kDa
Calculated molecular weight
245.1 kDa
Background of CACNA1D
Cav1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), is a human gene. It is mapped to 3p21.1. Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Alpha-1D subunit can mediate DHP-sensitive, high voltage-activated, long-lasting calcium channel activity. CACNA1D can form L-type calcium channels with negative activation thresholds which is essential for normal auditory function and controling of cardiac pacemaker activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2293 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-CaV1.3 antibody, PA2293, All Western blotting
All lanes: Anti-CACNA1D(PA2293) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 245KD
Observed bind size: 245KD
Specific Publications For Anti-CaV1.3/CACNA1D Antibody Picoband® (PA2293)
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7 Customer Q&As for Anti-CaV1.3/CACNA1D Antibody Picoband®
Question
Is a blocking peptide available for product anti-CaV1.3/CACNA1D antibody (PA2293)?
R. Jones
Verified customer
Asked: 2020-03-20
Answer
We do provide the blocking peptide for product anti-CaV1.3/CACNA1D antibody (PA2293). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-CaV1.3/CACNA1D antibody for WB for human pancreatic islet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pancreatic islet identification?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can see on the product datasheet, PA2293 anti-CaV1.3/CACNA1D antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreatic islet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
Do you have a BSA free version of anti-CaV1.3/CACNA1D antibody PA2293 available?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CaV1.3/CACNA1D antibody PA2293 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-19
Question
Would anti-CaV1.3/CACNA1D antibody PA2293 work for WB with pancreatic islet?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
According to the expression profile of pancreatic islet, CACNA1D is highly expressed in pancreatic islet. So, it is likely that anti-CaV1.3/CACNA1D antibody PA2293 will work for WB with pancreatic islet.
Boster Scientific Support
Answered: 2019-07-08
Question
Would anti-CaV1.3/CACNA1D antibody PA2293 work on feline WB with pancreas?
W. Banerjee
Verified customer
Asked: 2017-10-17
Answer
Our lab technicians have not validated anti-CaV1.3/CACNA1D antibody PA2293 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CaV1.3/CACNA1D antibody PA2293 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-17
Question
My question regarding product PA2293, anti-CaV1.3/CACNA1D antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Baker
Verified customer
Asked: 2015-05-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2293 anti-CaV1.3/CACNA1D antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-05-12
Question
I see that the anti-CaV1.3/CACNA1D antibody PA2293 works with WB, what is the protocol used to produce the result images on the product page?
G. Roberts
Verified customer
Asked: 2013-01-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-01-25
