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- Table of Contents
CCAR2 · Western blot design guide
CCAR2 Western Blot Planning Guide
Plan a CCAR2 Western blot around the catalog-observed 102.9 kDa band, image-backed A03412-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CCAR2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 102.9 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Cerebellum | |
| Negative control | Lung |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 102.9 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated CCAR2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bronchus |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03412-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CCAR2 Western Blot Band Size?
Use the product-observed 102.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 102.9 kDa | Matches the authoritative product WB observation. |
| 102.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CCAR2 appearancePlan around 102.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 102.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 102.9 kDa from UniProt Q8N163; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03412-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 102.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CCAR2 band
A03412-1 · CCAR2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CCAR2 Western blot
🧪Use Cerebellum as the first positive-control candidate and Lung as the HPA Not detected negative candidate.
Positive control: Cerebellum (High)
Negative control: Lung (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CCAR2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Esophagus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced CCAR2 Western Blot Tips
Deeper troubleshooting and optimisation questions for CCAR2, answered from its protein features.
Which band should guide the blot?
Use 102.9 kDa, the observation attached to the authoritative A03412-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 102.9 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Lung as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CCAR2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03412-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CCAR2 Western Blot Reagents
Human/Mouse/Rat-reactive CCAR2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-DBC-1/CCAR2 Antibody Picoband®
Cat # A03412-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q8N163
- Human Protein Atlas — CCAR2
- A03412-1 product record
- PMC5258681 — CCAR2 Is Required for Proliferation and Tumor Maintenance in Human Squamous Cell Carcinoma (The Journal of investigative dermatology, 2017)
- PMC10488071 — A Novel Long Noncoding RNA in Osteocytes Regulates Bone Formation through the Wnt/β-Catenin Signaling Pathway (International journal of molecular sciences, 2023)
- PMC10688608 — The gut microbiota reprograms intestinal lipid metabolism through long noncoding RNA Snhg9 (Science (New York, N.Y.), 2023)