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- Table of Contents
CCNG1 / Cyclin-G1 · Western blot design guide
Design a Western Blot for CCNG1
Source-linked CCNG1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CCNG1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CCNG1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~34.1 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adipose tissue (IHC candidate; verify WB) +4 more | |
| Negative control | Heart muscle (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked CCNG1 Western Blot Protocol Options
The A04847 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A04847; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected CCNG1 Western Blot Band Size?
Cyclin-G1 has a predicted mass of 34.1 kDa; alternative splicing could affect migration, but no empirical band size or distinct isoform pattern is supplied.
| Single band near 34.1 kDa | consistent with the predicted Cyclin-G1 mass; confirm identity with controls |
| Two bands at different positions | could reflect isoforms 1 and 2, but their migration is unknown |
| Band position varies between samples | could reflect different isoform expression; band identity needs confirmation |
| Weak whole-cell band with stronger nuclear signal | consistent with the reported nuclear location |
💡Expected CCNG1 appearanceUniProt predicts 34.1 kDa, but no empirical band size is supplied; isoforms 1 and 2 could differ in migration, so confirm any assigned band with identity controls.
| UniProt predicted mass | sets a 34.1 kDa reference, not a measured band position |
| Isoform 1 | has no supplied isoform-specific mass or migration |
| Isoform 2 | may differ in size from isoform 1, but its mass is not supplied |
| Alternative splicing | could change band position without establishing a resolvable pattern |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | nuclear Cyclin-G1 may be weak in the sampled whole-cell lysate | check a nuclear fraction and a validated positive control |
| Band higher than expected | the band's identity or an isoform-specific migration difference is unresolved | compare with a positive control and test signal loss after CCNG1 knockdown |
| Band lower than expected | the band's identity or an isoform-specific migration difference is unresolved | test signal loss after CCNG1 knockdown and assess isoform expression |
| Multiple bands | isoforms 1 and 2 are reported, but distinct bands are not established | check isoform expression and which bands respond to CCNG1 knockdown |
| Weak or no signal | the nuclear target may be underrepresented in the preparation | assess nuclear enrichment and sample loading |
Experiment evidence
Western blot images showing CCNG1 band
A04847 · CCNG1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CCNG1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CCNG1 in Western blot, you can use adipose tissue, which HPA scores High.
Positive control: Adipose tissue (IHC candidate; verify WB)
Negative control: Heart muscle (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: CCNG1 is nuclear, so a nuclear-enriched lysate may improve detection.
HPA tissue expression evidence for CCNG1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | High | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | High | Protein (IHC) | HPA → |
| Caudate | neuronal cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Liver | cholangiocytes | Not detected | Protein (IHC) | HPA → |
| Nasopharynx | ciliated cells (cell body) | Low | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | Low | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced CCNG1 Western Blot Tips
Deeper troubleshooting and optimisation questions for CCNG1, answered from its protein features.
What band size should I expect for CCNG1?
Band shift · The canonical 295-residue protein has a predicted mass of 34.1 kDa. No observed Western blot band is supplied, so use 34.1 kDa as a reference rather than an established apparent size.
Could CCNG1 isoforms produce different bands?
Isoforms · UniProt lists two isoforms. Isoform 2 lacks residues 1–134 of the canonical sequence, so it may migrate differently. These are UniProt coordinates; check the numbering convention used for any antibody epitope.
Check whether its epitope falls within canonical residues 1–134, which are missing in isoform 2. An antibody targeting that region would not be expected to detect isoform 2. Confirm epitope numbering against the UniProt sequence.
Check whether its epitope falls within canonical residues 1–134, which are missing in isoform 2. An antibody targeting that region would not be expected to detect isoform 2. Confirm epitope numbering against the UniProt sequence.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of CCNG1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CCNG1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04847 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify nuclear CCNG1?
Quantitation · Because CCNG1 is annotated as nuclear, compare consistently prepared nuclear samples with an appropriate loading reference. If both isoforms are detected, define whether quantitation includes both bands or evaluates each separately.
Does DNA damage imply higher CCNG1 signal?
Interpretation · UniProt places CCNG1 in the nucleus and notes its presence at DNA replication foci after DNA damage. That localization does not establish increased total protein abundance. Compare matched samples if assessing a DNA damage response.
Consider whether a smaller band could correspond to isoform 2, which lacks canonical residues 1–134, and check antibody epitope coverage. UniProt lists no modified residues or glycosylation sites here; the supplied features do not establish a modification-driven shift or identify any observed band.
Consider whether a smaller band could correspond to isoform 2, which lacks canonical residues 1–134, and check antibody epitope coverage. UniProt lists no modified residues or glycosylation sites here; the supplied features do not establish a modification-driven shift or identify any observed band.
Boster reagents
CCNG1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Cyclin G CCNG1 Antibody
Cat # A04847
A04847 is an anti-CCNG1 polyclonal antibody listed for Western blotting, with a WB validation image. The supplied caption mentions analysis of cells but does not identify the samples or provide complete gel conditions.
Which to pick: A04847 is the only listed CCNG1 antibody. Its stated reactivity is Human, Mouse and Rat, and it has a WB image; check the full validation details for your sample and conditions.
Source: BosterBio CCNG1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P51959.
- Human Protein Atlas. CCNG1 tissue expression.
- PMC7106816 — target-verified WB comparison
- PMC11544242 — target-verified WB comparison
- PMC3351588 — target-verified WB comparison