This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CCNG2 / Cyclin-G2 · Western blot design guide
Design a Western Blot for CCNG2
Source-linked CCNG2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CCNG2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CCNG2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~38.9 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Liver (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked CCNG2 Western Blot Protocol Options
The A09432 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A09432; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected CCNG2 Western Blot Band Size?
CCNG2 is predicted at 38.9 kDa; two isoforms could affect migration, but no distinct band pattern or empirical band size is established.
| Band near 38.9 kDa | Consistent with the predicted CCNG2 size; confirm identity with controls |
| Additional band at another size | Could reflect annotated isoform 1 or 2; their migration is not supplied |
| Single detectable band | The two annotated isoforms may not resolve or both be detected |
| Band in a cytoplasmic fraction | Consistent with CCNG2 cytoplasmic localization |
💡Expected CCNG2 appearanceCCNG2 has a predicted mass of 38.9 kDa, but no empirical band size is supplied; confirm a candidate band with antibody and expression controls because isoform migration is unknown.
| UniProt predicted mass | Places the predicted CCNG2 band near 38.9 kDa |
| Isoform 1 | Annotated splice isoform; its individual mass and migration are not supplied |
| Isoform 2 | Annotated splice isoform; its individual mass and migration are not supplied |
| Alternative splicing | May produce a different apparent size, but distinct bands are not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | CCNG2 signal may be below detection in the sampled lysate | Check cytoplasmic protein recovery and use an expression control |
| Band higher than expected | Its identity or migration is unverified; the supplied features establish no size-raising modification | Compare with CCNG2 knockdown or another validated antibody |
| Band lower than expected | An isoform is possible, but its mass is unknown | Check isoform expression and confirm band identity with knockdown |
| Multiple bands | Isoforms 1 and 2 are annotated, though distinct migration is unproven | Use knockdown and isoform-aware controls to assign bands |
| Weak or no signal | CCNG2 abundance in the sample is unknown | Check sample expression, loading, and antibody performance |
| Broad smear instead of sharp band | No supplied CCNG2 feature establishes a smear | Check sample quality and antibody specificity |
Experiment evidence
Western blot images showing CCNG2 band
A09432 · CCNG2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CCNG2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CCNG2 in Western blot, you can use adrenal gland tissue, which HPA rates as high expression.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Liver (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA reports no detectable CCNG2 in liver, making it a feasible negative tissue control.
HPA tissue expression evidence for CCNG2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | lymphoid tissue | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Nasopharynx | respiratory epithelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Liver | cholangiocytes | Not detected | Protein (IHC) | HPA → |
| Lung | alveolar cells | Not detected | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | smooth muscle cells | Not detected | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Low | Protein (IHC) | HPA → |
Section 3
Advanced CCNG2 Western Blot Tips
Deeper troubleshooting and optimisation questions for CCNG2, answered from its protein features.
What band size should I expect for CCNG2?
Band shift · The canonical CCNG2 sequence is 344 amino acids with a predicted mass of 38.9 kDa. No observed Western blot band position is supplied, so use 38.9 kDa as a calculated reference, not a guaranteed apparent size.
How does isoform 2 differ from canonical CCNG2?
Isoforms · In UniProt numbering, isoform 2 replaces canonical residues 304–344 with one arginine, making it 40 residues shorter. A lower apparent band is possible, but the sequence difference alone does not establish where either isoform migrates on a blot.
Check the antibody epitope against the UniProt sequences. Residues 304–344 of isoform 1 are replaced in isoform 2; an epitope in that region may distinguish them. An epitope in the shared sequence may recognize both, subject to antibody validation.
Possibly. Isoform 2 is 40 residues shorter because UniProt residues 304–344 are replaced by one arginine. Compare antibody epitope coverage and band behavior before assigning identity; the supplied features do not establish an observed band size.
Check the antibody epitope against the UniProt sequences. Residues 304–344 of isoform 1 are replaced in isoform 2; an epitope in that region may distinguish them. An epitope in the shared sequence may recognize both, subject to antibody validation.
Possibly. Isoform 2 is 40 residues shorter because UniProt residues 304–344 are replaced by one arginine. Compare antibody epitope coverage and band behavior before assigning identity; the supplied features do not establish an observed band size.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of CCNG2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CCNG2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A09432 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify CCNG2 if two bands appear?
Quantitation · Decide whether the measurement is total CCNG2 or an individual isoform. Quantify bands separately when assessing isoforms, and verify antibody recognition before interpreting their relative intensities: the supplied features do not establish equal detection of isoforms 1 and 2.
Which sample fraction is relevant for CCNG2 detection?
Interpretation · UniProt lists CCNG2 in the cytoplasm. A cytoplasmic fraction is therefore a reasonable sample to examine. Check fraction quality when interpreting a weak or absent signal.
Boster reagents
CCNG2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Cyclin-G2 CCNG2 Antibody
Cat # A09432
The catalog reports anti-CCNG2 antibody A09432 for Western blot, with stated human and mouse reactivity. Its WB image shows KB cell lysates using primary antibody at 1:1000 overnight at 4°C. This example does not establish performance across all human or mouse samples.
Which to pick: A09432 is the only listed CCNG2 antibody. It has a WB image from KB cell lysates; use the reported 1:1000 overnight incubation at 4°C as a starting point, and verify performance in your own samples.
Source: BosterBio CCNG2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q16589.
- Human Protein Atlas. CCNG2 tissue expression.
- PMC12486569 — target-verified WB comparison
- PMC5915460 — target-verified WB comparison
- PMC7521070 — target-verified WB comparison