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- Table of Contents
Facts about Cadherin-12.
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Human | |
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Gene Name: | CDH12 |
Uniprot: | P55289 |
Entrez: | 1010 |
Belongs to: |
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No superfamily |
Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2
Mass (kDA):
88.332 kDA
Human | |
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Location: | 5p14.3 |
Sequence: | 5; NC_000005.10 (21750673..22853344, complement) |
Brain.
Cell membrane; Single-pass type I membrane protein.
Scientists may want to know how to best use the CDh22Marker in their research. This marker can be used to help you find the answers that you need, whether you are researching a specific species or an application. Boster Bio offers product credits for scientists using the marker for research. In addition to scientists in the U.S., Boster scientists are also welcome to submit their results worldwide.
The Anti-N-Cadherin-CD325-antibody from Boster Bio recognizes cadherin-2, a transmembrane protein that plays a role in cell-cell adhesion. The protein's central region, the C-terminal, interacts with catenin to mediate cell adhesion. The protein is present in tissues of mesodermic origin. It is also involved cell-cell division and migration, and promotes apoptosis.
This monoclonal antibody binds human N-Cadherin. It is a 130-kDa molecular weight (MDA) protein that contains five extracellular repeats, one transmembrane domain and cytoplasmicdomains. CD325-deficient mice can cause major defects in the heart and malformed development of the neural tube. Studies have shown that this protein is important in cardiac development as well as left-right and looping patterns.
The Boster Bio Anti-N-Cadherin-CD325-Antibody binds Human, Mouse, Rat, and rat CD315. It does NOT cross-react or interact with E-Cadherin. The N-Cadherin-2/CD325-Antibody can be used in western blots and is designed to detect bands of 130-kDa.
Boster Bio's antiBR-Cadherin-12 antibody (CDh22 marker) is an epitope specific antibody that binds directly to the first intron in human ectopic CDh22 genetic. The TGACCA motif can be found in 982 bp downstream from the transcriptional start site and 10.2 kb ahead of the first protein-coding ectopic exon. It is surrounded by a 55-bp-long conserved region that is highly conserved across species. The anti-BRCadherin-12 marker has been validated using chromatin immunoprecipitation as well as quantitative real-time PCR (a primer pair flanking it).
This protein is an excellent candidate to study the functions cadherins play in cellular attachment. The protein was found to be mainly expressed in the brain, suggesting that it plays a part in neuronal formation. It has been linked to synaptogenesis in our nervous system. Cadherin plays a crucial role in cell-cell adhesion regulation, regardless of its function.
Boster Bio has a high quality anti-BR-Cadherin-12 anti-body to detect the presence this protein on cells and tissues. This antibody reacts with human and mouse cells. This antibody is a great choice. It is compatible and available in a variety BR-Cadherin-12 (CDh22), antibody kits.
This antibody recognizes many types of tumor cells. It can capture nearly 90% tumor cells, regardless what their state is. It does not express these markers on tumor cells that have lower levels of EpCAM or CaLu1 expression, even though they are considered mesenchymal in culture. It is still a powerful anti-BR-Cadherin-12 anti-abwehr.
The methods described in this invention can monitor the progression of cancer in a subject. In a subject who has no tumor, the methods of the invention can be repeated over a period of time. The appearance of surface cells containing cadherin indicates that the subject is suffering from cancer. In addition, a benign tumor may not contain CTCs at baseline, and has therefore become metastatic and malignant.
Two determinants are responsible for lateral adhesion and cadherin type specific adhesion in the EC1 domain. These determinants are exposed in the EC1 domain. E-cadherin, EC1 and E2-cadherin are short-lived. This is an important role for the formation of the adhesion protein compound.
The CDh22 mark is a recombinant, fusion protein that corresponds to the amino acids 390-540 of human CDh22. The antibody can be used in a 1,000,000 dilution in blocking buffer (33% nonfat dry milk, TBST). This antibody is not intended to be used for diagnostic or therapeutic purposes. The following are examples of CDh22 markers. Let's look at each of them individually.
PMID: 7982033 by Tanihara H., et al. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin.
PMID: 7731968 by Selig S., et al. Expressed cadherin pseudogenes are localized to the critical region of the spinal muscular atrophy gene.