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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cdh12 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2068)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L18-A716
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2068 is reactive to Cdh12 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK2068 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cadherin-12
Cadherin-12 is a protein that in humans is encoded by the CDH12 gene. It is mapped to 15 A2; 15 8.25 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. This gene is expressed in both the excitatory and inhibitory neurons in various areas of the adult central nervous system including the cerebellum, neocortex, hippocampus and in different subcortical nuclei. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 15.
|EK2068-CAP||Anti-Mouse Cdh12 Pre-coated 96-well strip microplate||1|
|EK2068-ST||Mouse Cdh12 Standard||2 vials, 10 ng/tube|
|EK2068-DA||Mouse Cdh12 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse Cadherin-12 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cadherin-12, Dilution ratio of 1:50, concentration in serum is 60-130ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Cadherin-12 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cdh12 (Source: Uniprot.Org, NCBI)
Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cdh12, check out the Cdh12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cdh12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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