Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine®

Cadherin-12 ELISA kit for Mouse

Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cdh12 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2068
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

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Product Name

Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine®

View all Cadherin-12 ELISA kits

SKU/Catalog Number

EK2068

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cdh12 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2068)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-A716

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2068 is reactive to Cdh12 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2068 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cadherin-12

Cadherin-12 is a protein that in humans is encoded by the CDH12 gene. It is mapped to 15 A2; 15 8.25 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. This gene is expressed in both the excitatory and inhibitory neurons in various areas of the adult central nervous system including the cerebellum, neocortex, hippocampus and in different subcortical nuclei. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 15.

Kit Components

Catalog Number Description Quantity
EK2068-CAP Anti-Mouse Cdh12 Pre-coated 96-well strip microplate 1
EK2068-ST Mouse Cdh12 Standard 2 vials, 10 ng/tube
EK2068-DA Mouse Cdh12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0380.1470.2430.4070.8031.1451.9142.250

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cadherin-12, Dilution ratio of 1:50, concentration in serum is 60-130ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)169933297618310164238
Standard deviation7.1040.12122.0212.0873.15322.09
CV (%)4.2%4.3%4.1%6.6%7.2%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cadherin-12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11691801861711766.873.9%
Sample 293388294186490532.743.6%
Sample 32976319530343038306081.292.6%
*number of samples for each test n=16.

Gene/Protein Information For Cdh12 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q5RJH3

Gene ID

215654

Gene Name

Cdh12

Full Name

Cadherin-12

Weight

88.468kDa

Alternative Names

Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cdh12, check out the Cdh12 Infographic

Cdh12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cdh12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine®

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-10-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Cadherin-12 using the EK2068 Mouse Cadherin-12 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-07-01

Question

Q: how to analyze ELISA data? I measured Cadherin-12 level in serum.

Verified Customer

Verified customer

Asked: 2019-06-30

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-06-30

Question

Q: What is the optimal O.D. value for Cadherin-12 ELISA kit? I used your Cadherin-12 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cadherin-12 even though the O.D. values are not very high?

W. Varma

Verified customer

Asked: 2019-01-11

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cadherin-12 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Cadherin-12 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2019-01-11

Question

Q: which procedure should I follow in order to thaw whole blood sample for Cadherin-12 ELISA after freezing?

M. Dayal

Verified customer

Asked: 2018-07-02

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cadherin-12 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-07-02

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

S. Green

Verified customer

Asked: 2017-03-14

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-03-14

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

P. Hall

Verified customer

Asked: 2016-10-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-10-02

Question

Q: if the enzyme conjugated Cadherin-12 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cadherin-12 antigen?

W. Mehta

Verified customer

Asked: 2016-05-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-30

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