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- Table of Contents
CEL / Bile salt-activated lipase · Western blot design guide
Design a Western Blot for CEL
Real validated CEL Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CEL WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CEL Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~79.3 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Pancreas (IHC candidate; verify WB) +2 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Cleaved | |
| Caveat | Glycosylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated CEL Western Blot Protocols
The A01821 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cell lines, (catalog A01821) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01821 · 1:500 (catalog A01821) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL (catalog A01821) |
Section 2
What Is the Expected CEL Western Blot Band Size?
UniProt predicts a 79.3 kDa precursor; secretion, signal-peptide cleavage, glycosylation, and isoforms could affect the blot, but no migration position is demonstrated.
| Band near 79.3 kDa | consistent with the predicted precursor mass, pending identity confirmation |
| Band above 79.3 kDa | could reflect glycosylation, but its migration effect is unmeasured |
| Band below the precursor position | could reflect removal of the 1–20 signal peptide |
| Little or no band in whole-cell lysate | consistent with secretion of CEL |
| Several bands at different positions | could include Long and Short isoforms; their separation is unknown |
| Broad band or smear | could reflect heterogeneous glycosylation across the annotated sites |
💡Expected CEL appearanceUniProt predicts a 79.3 kDa precursor; signal-peptide cleavage, glycosylation, and Long and Short isoforms could alter migration, but no empirical band size is supplied, so confirm band identity with appropriate controls.
| UniProt predicted precursor mass | sets a 79.3 kDa sequence-based reference, not a validated migration position |
| Complex N-linked glycosylation at Asn207 | could increase apparent size; the shift is not quantified |
| O-linked GalNAc sites at Thr558, Thr569, Thr579, Thr607, Thr618, Thr629, and Thr640 | could affect apparent size or band breadth; neither effect is established |
| Long and Short isoforms | may differ in size, but their masses and separation are not supplied |
| Signal peptide at residues 1–20 | cleavage yields a smaller mature chain than the precursor |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | CEL is secreted | check conditioned medium alongside lysate and include a positive control |
| Band higher than expected | annotated N- and O-linked glycosylation could affect migration | compare with an appropriately deglycosylated sample and confirm band identity |
| Band lower than expected | removal of the 1–20 signal peptide could lower mass | compare secreted and cellular samples and confirm the detected band with an independent antibody |
| Broad smear instead of sharp band | heterogeneous glycosylation across annotated sites is possible | compare with an appropriately deglycosylated sample |
| Multiple bands | Long and Short isoforms or different glycosylation states are possible | compare isoform-specific controls if available and test glycosylation sensitivity |
| Weak or no signal | secreted CEL may be scarce in the sampled lysate | test conditioned medium and a CEL-positive sample |
Experiment evidence
Western blot images showing CEL band
A01821 · CEL
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CEL Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CEL in Western blot, you can use pancreas tissue lysate.
Positive control: Pancreas (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside.
⚠️Feasibility: CEL is secreted, so lysate signal may be limited; consider the secreted fraction.
HPA tissue expression evidence for CEL
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Pancreas | exocrine glandular cells | High | Protein (IHC) | HPA → |
| Lactating breast | lactating glandular cells | Medium | Protein (IHC) | HPA → |
| Stomach | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CEL Western Blot Tips
Deeper troubleshooting and optimisation questions for CEL, answered from its protein features.
How should CEL band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could the Long and Short isoforms produce different bands?
Isoforms · UniProt lists Long and Short isoforms; Short lacks residues 430–495. Compare bands with the antibody’s target region in mind: an antibody recognizing that interval may miss Short. The sequence difference can affect mass, but the supplied features do not establish where either isoform migrates.
Which CEL glycosylation sites matter when interpreting band patterns?
PTM · UniProt annotates N-linked glycosylation at Asn207 and O-linked glycosylation at Thr558, 569, 579, 607, 618, 629, 640, 651, 662 and 673. These are UniProt sequence coordinates; paper or antibody numbering may differ. Glycosylation is relevant to band interpretation, but the site list alone does not establish distinct visible bands.
Does this guide establish induction of CEL?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CEL?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01821 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should CEL bands be quantified across samples?
Quantitation · Use a consistent sample fraction and decide whether the measurement targets one band or all validated CEL bands. CEL is secreted and has Long and Short isoforms, so changing fractions or combining bands without identifying them can change what the measurement represents. The supplied features provide no reference band position.
Why might CEL migrate differently from its predicted 79.3 kDa?
Interpretation · The 79.3 kDa prediction is for the supplied sequence. CEL has a signal peptide at residues 1–20 and 11 annotated glycosylation sites, both relevant when interpreting apparent mass. These features alone cannot predict a visible shift, and no observed band size was supplied.
CEL is annotated as secreted, with a signal peptide at residues 1–20. Include an appropriate extracellular sample when assessing its expression; a cell lysate alone may not represent the secreted pool. Interpret the two fractions separately if both are measured.
Check whether a band is consistent with the Long or Short sequence, including Short’s missing residues 430–495, and whether the antibody recognizes that interval. Also consider the signal peptide and annotated glycosylation when assessing apparent mass. These features suggest checks; they do not identify an unexpected band by themselves.
UniProt lists two disulfide bonds. Compare reducing and nonreducing preparations if band behavior differs between protocols, while keeping the sample fraction consistent. The supplied features do not establish a particular migration pattern under either condition.
CEL is annotated as secreted, with a signal peptide at residues 1–20. Include an appropriate extracellular sample when assessing its expression; a cell lysate alone may not represent the secreted pool. Interpret the two fractions separately if both are measured.
Check whether a band is consistent with the Long or Short sequence, including Short’s missing residues 430–495, and whether the antibody recognizes that interval. Also consider the signal peptide and annotated glycosylation when assessing apparent mass. These features suggest checks; they do not identify an unexpected band by themselves.
UniProt lists two disulfide bonds. Compare reducing and nonreducing preparations if band behavior differs between protocols, while keeping the sample fraction consistent. The supplied features do not establish a particular migration pattern under either condition.
Boster reagents
CEL Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Bile salt-activated lipase CEL Antibody
Cat # A01821
A01821 is an anti-CEL antibody listed as reactive with human, mouse, and rat samples. Its Western blot image uses extracts from various cell lines at 1:500 dilution; the caption does not identify the cell lines or establish species-specific validation.
Which to pick: A01821 is the only listed option and has a Western blot image. The reported blot used 25 µg of cell-line lysate per lane and a 1:500 primary antibody dilution.
Source: BosterBio CEL gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P19835.
- Human Protein Atlas. CEL tissue expression.
- PMC9843195 — target-verified WB comparison
- PMC7663422 — target-verified WB comparison