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- Table of Contents
Facts about Centrosomal protein of 55 kDa.
Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290).
Human | |
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Gene Name: | CEP55 |
Uniprot: | Q53EZ4 |
Entrez: | 55165 |
Belongs to: |
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No superfamily |
C10orf3; cancer/testis antigen 111; centrosomal protein 55kDa; centrosomal protein of 55 kDa; Cep55; chromosome 10 open reading frame 3; CT111; FLJ10540; Up-regulated in colon cancer 6; URCC6
Mass (kDA):
54.178 kDA
Human | |
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Location: | 10q23.33 |
Sequence: | 10; NC_000010.11 (93496612..93529094) |
Expressed in embryonic brain (PubMed:28264986). Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986). Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28295209, PubMed:28264986). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cleavage furrow. Midbody, Midbody ring. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mi
Anti-CEP55 markers can be used if you plan to conduct molecular biology research. These markers can be used to identify and detect C-terminal antigens. Boster Bio is the manufacturer of these antibodies. Boster Bio has been in operation since 1993. It is best known for its ELISA products. It recently began to offer PCR-related molecular biology products. The company offers many services to its customers, including technical support that is available 24 hours a day and free downloadable technical resources.
This boster bio-antibody is specific for the centrosomal protein 55 kD. CEP55 is a protein that is highly expressed in human gastric and prostate cancers. CEP55 expression can promote cell migration and infiltration. The link below will allow you to see detailed information about the product.
CEP55 is an essential component of primary cilia that protrude from the cell surface and have a variety of roles during development and disease. The inactivation of the assembly process causes ciliopathy, and diseases resulting from disassembly dysfunction are still unknown. CEP55 promotes the disassembly of cilia in mice, and mice lacking Cep55 exhibit clinical signs such as Meckel-Gruber syndrome or abnormal tissue elongation. This protein interacts with Aurora A kinase, a component of the ciliary system that promotes cilia disassembly.
These reagents, which are also available to scientists, are readily available around the globe. Scientists can use them for research purposes, and they can submit results related to applications and special samples for product credits. The company's ELISA kit can detect biomarkers for cancer, neurosciences, developmental biology, and other diseases. They can be sensitive to a picogram level. They are accessible through tebubio.
Researchers used the abbreviated Gene Sequence ACC to assess the anti-Centrosomal Protein of 55kDa CEP55. Then they used the BosterBio Antientrosomal protof of 55 kDa CEP55 anti-Centrosomal antibody to identify specific antibodies. The amplification program was repeated for 40 cycles, and the results were compared to the GAPDH housekeeping gene using the 2-DDCT method.
The CEP55 gene marker plays a crucial role in brain developmental. The gene activates PI3K, which promotes cell growth. This gene is also essential for cytokinesis which is an essential process in cell division. CEP55 can be used to identify neural stem cell and to understand the mechanisms behind cell division. However, these applications are limited by the lack of functional information on the role of Cep55 in adult intestine.
The CEP55 protein encodes a 464 amino-acid protein. It is located in the centrosome during interphase as well as throughout mitosis. To perform its function, the gene is a homodimer. Tumor progression can be caused by defects in this gene. This marker can be used to detect malignant cells and aid in cancer research.
The conditions that affect the expression of a gene are crucial. It is crucial to identify gene fusions in cancer. These cells are more likely than others to develop into cancer. A gene can also cause cell changes. For example, one mutation in the CEP55 gene may cause the cell to transform into a cancerous one. If this mutation is found in the intestine, the CEP55 gene may need to be altered.
CEP55 activates the pathway PI3K/AKT/mTOR. CEP55 could therefore play an important part in RCC. In the future, this gene may also serve as an effective prognostic marker. CEP55 is a gene that promotes tumor growth. It can also promote cell migration and invasion. These are just some of the benefits of using CEP55 in cancer research. There are many applications for CEP55, so make sure to explore these genes.
CEP55 is a promising prognostic marker for patients with HCC. CEP55 promotes tumor development by activating the PI3K/AKT pathway. HCC patients could benefit from targeting this pathway as a potential therapeutic option. Below are some benefits of the CEP55 marker for HCC. However, CEP55 is not a viable option in all cases.
Many human cancers have the CEP55 mutation, including ovarian, colon, and hepatocellular. It is expressed in many types of cancer cells, and it has many functions in cells. It is also important in HCC prognosis. CEP55's clinical benefits include many potential clinical applications.
CEP55 is involved in many aspects, including midbody morphology and vesicle transportking. This gene is a candidate to be mutated because it is essential for many biological processes, including cell cytokinesis. This gene also plays an important role in regulating the trafficking of vesicles. This gene can be mutated and cause a variety neurologic disorders.
Cep55 is required for cytokinesis. It is also associated with the spindle middlezone during mitosis, cytokinesis, and cytokine. Cells that lack Cep55 have decreased cell motility and failure to abscise. This defect may be caused by mislocalization of p38MAPK and MKLP2. Although Cep55 and these proteins do not interact during cytokinesis there is a direct interaction. Cell abscission is possible through the SNAREs MKLP2 & PRC1.
Researchers recently discovered that Cep55 and centralspindlin form a complex when cells undergo cytokinesis. The peak kinetics of the complex formed coincided with these times in cytokinesis. This suggests that Cep55 is controlled by centralspindlin during cytokinesis.
In vitro studies showed that recombinant Cep55 was able to associate with microtubules in vitro. It also binds MKLP1 or the MgcRacGAP protein complex. In this experiment GST was purified via SDS-PAGE. The GST-Cep55 samples were stained with Coomassie. The microtubules that had been purified were then pelleted with a glycerol pad, and immunofluorescence staining revealed tubulin.
In vitro production of FlagCep55 was also demonstrated in another experiment. The complexes of the proteins were immunoblotted, and Western blotted. Both experiments showed that the ratio of specific IP/non-specific IP indicated whether the cells were in anaphase, or cytokinesis. This indicates that Flag-Cep55, MgcRacGAP, and Flag-Cep55 co-reactive.
This study investigated the sensitivity and specificity of the CEP55 marker for the detection of prostate carcinoma. This marker can be expressed both in one or two forms. Because of alternative splicing events, CEP55 can have either a high or low expression. CEP55 expression was therefore assessed by immunohistochemistry and semiquantitative RTPCR. These results were consistent with an earlier published study.
CEP55 expression in cancer patients has been linked to poor prognosis. However, no studies have yet shown a direct relationship between CEP55 levels and survival rates at ESCC. This study was conducted to examine the CEP55 marker's sensitivity in cancer tissues and the relationship it has to patient survival following Ivor Lewis esophagectomy.
ESCC is a disease characterized by alterations in multiple genes and corresponding proteins. The centrosomal proteins 55 (CEP55), which shares many properties with oncogenes and is often overexpressed in malignant tumors, has a number of common properties. This study assessed CEP55 expression in patients suffering from mid-thoracic ESCCC. Immunohistochemistry and semi-quantitative reverse transcription-polymerase chain reaction were used to assess CEP55 expression in tumour tissue and healthy esophageal mucosa.
CEP55, a protein found in many types cancers, is highly expressed. It plays an essential role in the progression tumors. Knockdown of CEP55 in RCC inhibits cellular proliferation, migration, and invasion. On the other hand, overexpression of CEP55 enhances the growth of tumor cells and promotes the onset of epithelial-mesenchymal transition in RCC.
CEP55 is not a diagnostic indicator, but studies have shown that it can help in diagnosing and prognosis for RCC. It promotes EMT via PI3K/AKT/mTOR pathways and may even serve as a prognostic marker. However, the research has not been conclusive. CEP55 is a diagnostic tool that can be used in clinical practice. However, there are many questions and challenges. If used correctly, it could prove to be a valuable tool for patients who have been diagnosed with the disease.
PMID: 16406728 by Martinez-Garay I., et al. The novel centrosomal associated protein CEP55 is present in the spindle midzone and the midbody.
PMID: 16198290 by Fabbro M., et al. Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis.