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CKAP5 / Cytoskeleton-associated protein 5 · IHC design guide
Design Immunohistochemistry for CKAP5
Plan CKAP5 chromogenic IHC in paraffin sections using cytoplasmic staining as the expected tissue pattern (HPA tissue IHC). This guide covers an IHC-validated antibody concentration of 2–5 μg/mL (datasheet A05324) and mitotic spindle localization as a molecular expectation (UniProt).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
CKAP5 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining in tissue IHC (HPA tissue IHC) | |
| Staining pattern | Widespread cytoplasmic staining, including cortical neurons (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet A05324) | |
| Positive control | Adipose tissue+4 more · see all | |
| Negative control | None in HPA (detected in all 45 tissues); use no-primary + isotype controls |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep paraffin-section fixation consistent (standard IHC practice; not target-specific) | |
| Caveat | Mitotic spindle localization may alter the pattern (UniProt) | |
| Regulation | Elevated in hepatomas and colonic tumors (UniProt) | |
| Isoform / epitope | 3 isoforms; verify epitope coverage for each (UniProt) |
Section 1
Recommended CKAP5 IHC & IF Protocols
The catalog antibody’s IHC-P protocol uses EDTA pH 8.0 retrieval (datasheet A05324). One published xenograft tumor protocol uses acidic retrieval (PMC10075965).
| Sample | Paraffin-embedded human liver cancer tissue; fixative not specified (datasheet A05324) |
| Fixation | Image fixative and duration unreported (datasheet A05324); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet A05324); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A05324) |
| Primary antibody | Rabbit anti-CKAP5, 2-5μg/ml (datasheet A05324) |
| Primary incubation | Overnight at 4 °C (datasheet A05324) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet A05324) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | CKAP5-positive staining in adipocytes of adipose tissue (HPA tissue IHC: High). HPA tissue profile: Ubiquitous cytoplasmic expression. No signal in the no-primary control. |
💡Decision noteStart with EDTA pH 8.0 heat retrieval for the catalog antibody (datasheet A05324); use 20 min acidic retrieval when reproducing the published protocol (PMC10075965).
Section 2
What Is the Expected CKAP5 Staining Pattern?
CKAP5 is a cytoplasmic microtubule protein associated with centrosomes, spindle poles, spindles and kinetochores (UniProt Q14008). In paraffin sections, expect cytoplasmic staining in many cell types; HPA describes ubiquitous cytoplasmic expression and rates its tissue IHC evidence as Supported, with medium consistency between staining and RNA data (HPA tissue IHC). CKAP5 has no transmembrane segment (UniProt Q14008 topology).
| Cytoplasmic staining appears across several tissues, with stronger signal in selected cells. | This fits HPA’s ubiquitous cytoplasmic profile and low tissue RNA specificity (HPA tissue IHC). High staining is reported in adipocytes, cerebral cortex neurons and several glandular cell populations (HPA tissue IHC). Judge intensity within the cell type being examined; ubiquitous expression does not imply equal staining in every cell (HPA tissue IHC). |
| Small focal structures are visible within cytoplasm, especially in dividing cells. | Centrosomal and mitotic structures are biologically plausible: CKAP5 localizes to centrosomes, spindle poles, spindles and kinetochores (UniProt Q14008). HPA supports centrosome localization by ICC-IF; its additional mitotic spindle annotation is uncertain (HPA subcellular). Limited resolution in chromogenic sections can make these structures difficult to distinguish (general IHC practice). |
| Staining is confined to nuclei or outlines cell membranes without convincing cytoplasm. | An exclusive pattern there does not match HPA’s tissue IHC cytoplasmic profile or CKAP5’s lack of a transmembrane segment (HPA tissue IHC; UniProt Q14008 topology). Treat it as suspect and compare with controls. HPA also lists nucleolar and plasma membrane signals in ICC-IF as uncertain, so these observations alone cannot establish specificity (HPA subcellular). |
| One unexpected cell population stains much more strongly than the surrounding tissue. | Check the cell identification and controls before calling cross-reactivity or endogenous detection activity (general IHC practice). HPA reports high staining in colon endothelial cells, bronchial ciliated cell bodies and duodenal glandular cells (HPA tissue IHC). Its profile is broadly cytoplasmic and lists no negative cell population, so an unlisted positive cell is not automatically false (HPA tissue IHC). |
| Color is spread uniformly over cells and surrounding section, obscuring boundaries. | Poorly localized staining is hard to score against the reported cytoplasmic pattern (HPA tissue IHC). Assess a negative reagent control and background outside cells; incomplete blocking, detection background or excess primary antibody can contribute to diffuse signal (general IHC practice). Do not assign a CKAP5 compartment from uniform color alone. |
💡Expected CKAP5 appearanceCall a section positive when identifiable cells show cytoplasmic staining, potentially with focal centrosomal or mitotic structures; high signal is documented in adipocytes and cerebral cortex neurons, while exclusive chromatin or membrane outlines warrant control checks (HPA tissue IHC; HPA subcellular; UniProt Q14008).
| Tissue and cell selection | HPA reports High staining in adipocytes, adrenal glandular cells, bronchial ciliated cell bodies, cerebral cortex neurons, cervical and duodenal glandular cells, and colon endothelial cells; endometrial glandular cells are Medium (HPA tissue IHC). These are observed levels, not guaranteed control outcomes in every preparation (HPA tissue IHC). |
| Subcellular and cell-cycle context | CKAP5 participates in microtubule organization and localizes to centrosomes and mitotic spindle structures (UniProt Q14008). HPA’s supported ICC-IF location is the centrosome; basal body and additional locations are uncertain (HPA subcellular). A section with few mitotic cells may offer little opportunity to assess spindle staining (general IHC practice). |
| Antibody evidence | Both listed rabbit polyclonal antibodies, HPA039377 and HPA040375, have Supported IHC status; only HPA040375 has Supported ICC status in the supplied antibody record (HPA antibodies). Validation status supports using the observed pattern as a comparison, but does not establish that every stained structure is specific (HPA antibodies). |
| Molecular form and epitope | UniProt lists three isoforms, one chain spanning residues 1–2032, no signal peptide, no propeptide, no transmembrane segment and no annotated glycosylation sites (UniProt Q14008). Those annotations do not identify the catalog antibody’s epitope or establish isoform-specific staining or a fixation effect (UniProt Q14008). |
| IF/ICC: what pattern should be expected? | In the separate IF/ICC application, centrosome signal is supported; basal body, nucleolar, membrane, spindle and primary cilium annotations have uncertain status (HPA subcellular). UniProt also places CKAP5 at centrosomes, spindle poles, spindles and kinetochores (UniProt Q14008). Interpret those location calls within IF/ICC rather than treating them as required chromogenic IHC features. |
| Situation | Likely cause | Next action |
|---|---|---|
| A high-staining reference tissue gives no visible cell-associated signal. | Target-specific fixation effects are not established by the supplied assay evidence. Verify with a matched IHC source before attributing a result to fixation. | Inspect section integrity and counterstain, confirm the IHC reagent and detection controls, then follow the antibody’s documented IHC-P procedure if available (general IHC practice). Use an HPA High cell population as a reference, without treating its listed level as a guaranteed result (HPA tissue IHC). |
| Only nuclear chromatin or sharp cell borders stain. | This distribution conflicts with the predominant cytoplasmic tissue profile; uncertain ICC-IF nucleolar or membrane annotations do not validate an exclusive IHC pattern (HPA tissue IHC; HPA subcellular). | Review morphology and negative reagent controls, and compare another supported IHC antibody if available (general IHC practice; HPA antibodies). Score CKAP5 only where cell-associated staining is credible against the documented cytoplasmic pattern (HPA tissue IHC). |
| Many unexpected cells are positive. | CKAP5 has low tissue RNA specificity and broadly cytoplasmic tissue staining, while the supplied HPA list contains no negative cell population (HPA tissue IHC). An unexpected cell type alone cannot distinguish true signal from cross-reactivity. | Identify stained cells on the counterstained section and compare their compartment with HPA’s profile; use appropriate reagent controls to assess nonspecific detection (HPA tissue IHC; general IHC practice). |
| The entire section has diffuse color. | Background from staining or detection can obscure the cell-associated cytoplasmic pattern (general IHC practice; HPA tissue IHC). | Check a negative reagent control, wash and blocking performance, and the primary antibody concentration using its documented IHC-P instructions (general IHC practice). Reassess only after cellular boundaries and cytoplasm can be distinguished. |
| Cytoplasm stains, but no centrosomal puncta or spindle structures are apparent. | The HPA tissue IHC profile is cytoplasmic; centrosome support comes from ICC-IF, and mitotic structures depend on cells being in mitosis (HPA tissue IHC; HPA subcellular; UniProt Q14008). Chromogenic section resolution may limit recognition (general IHC practice). | Accept credible cytoplasmic staining as the tissue-level pattern; assess focal structures only where morphology permits (HPA tissue IHC; general IHC practice). Consult the separate IF/ICC application for its supported centrosome localization (HPA subcellular). |
| Signal differs between two IHC antibodies. | Both listed antibodies have Supported IHC status, while HPA describes only medium consistency between staining and RNA data; the supplied record gives no epitope mapping to resolve a difference (HPA antibodies; HPA tissue IHC). | Compare the same cell types and compartments across matched sections, then review each antibody’s documented IHC-P conditions and controls (general IHC practice). Report unresolved differences without assigning them to an isoform or fixation effect (UniProt Q14008; HPA tissue IHC). |
Experiment evidence
IHC & IF images showing CKAP5 staining
A05324 · IHCIHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). ch TOG/CKAP5 was detected in a paraffin-embedded section of human li
A05324 · IHCIHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). ch TOG/CKAP5 was detected in a paraffin-embedded section of human pr
A05324 · IFIF analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). ch TOG/CKAP5 was detected in an immunocytochemical section of A431 ce
M05324 · IHCIHC analysis of Ch TOG/CKAP5 using anti-Ch TOG/CKAP5 antibody (M05324). Ch TOG/CKAP5 was detected in paraffin-embedded section of human live
M05324 · IHCIHC analysis of Ch TOG/CKAP5 using anti-Ch TOG/CKAP5 antibody (M05324). Ch TOG/CKAP5 was detected in paraffin-embedded section of human rect
M05324 · IFIF analysis of Ch TOG/CKAP5 using anti-Ch TOG/CKAP5 antibody (M05324). Ch TOG/CKAP5 was detected in an immunocytochemical section of Hela ce
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000175216-CKAP5/tissue/cerebral+cortex#imid_10442957
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000175216-CKAP5/tissue/colon#imid_10442875
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000175216-CKAP5/tissue/liver#imid_10442884
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000175216-CKAP5/tissue/kidney#imid_10442893
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA040375; green = CKAP5, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA040375; green = CKAP5, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for CKAP5 IHC & IF
🧪Run bronchus first; ciliated cell bodies should stain (HPA: High in bronchial ciliated cell bodies). HPA detects CKAP5 in all 45 scored tissues, so no negative tissue is available: use no-primary and isotype controls, and treat cells on the positive slide that show only counterstain as background references rather than validated CKAP5-negative cells (HPA: no negative rows).
Positive control tissue: Adipose tissue (Adipocytes, HPA High)
Negative control tissue: None in HPA: CKAP5 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead.
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show CKAP5 in A-431, U-251MG, U2OS, ASC52telo, RPTEC/TERT1, hTERT-RPE1 (serum starved), with annotated localisation: Centrosome (supported), Basal body (uncertain) (HPA subcellular).
Technical controls: Use a no-primary, secondary-only control, a nonimmune rabbit IgG control matched to the primary’s clonality where known, and CKAP5 knockout material processed in parallel as a biological negative (caption: rabbit primary; standard IHC control practice). Quench endogenous peroxidase and check bronchial background before interpreting brown signal (caption: HRP/DAB detection; standard IHC practice).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected A05324 paraffin-section caption does not state a fixative (caption: fixative not stated). Heat retrieval in EDTA at pH 8.0 was used in that example, but a strict retrieval dependency and any frozen-section advantage are unreported (caption: EDTA retrieval). IF/ICC images exist, though relative ease is unestablished; in bronchus, score ciliated cell bodies rather than luminal material (HPA: ICC-IF images; HPA: High in bronchial ciliated cell bodies).
HPA tissue IHC evidence for CKAP5
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Supported — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | High | Protein (IHC) | HPA → |
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | Ciliated cells (cell body) | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
| Cervix | Glandular cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| None in HPA: CKAP5 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead. | ||||
Section 3
Advanced CKAP5 IHC Tips
CKAP5 staining is mainly cytoplasmic in tissue sections (HPA tissue IHC); its centrosomal and spindle associations help interpret finer localisation (UniProt Q14008).
Which retrieval conditions should I start with for CKAP5 in paraffin sections?
Start with heat-mediated retrieval in EDTA at pH 8.0 for the catalog antibody (datasheet A05324). That condition was used on a paraffin-embedded human liver cancer section stained with this antibody; the caption does not state the fixative or heating duration (datasheet A05324). If staining is weak, check that sections remained covered by retrieval buffer and compare a controlled change in heating time on adjacent sections while holding detection conditions constant (standard IHC practice). Compare signal in intact tissue with a no-primary control, because stronger DAB alone cannot establish that retrieval improved specific CKAP5 staining (standard IHC practice).
How should I troubleshoot weak CKAP5 staining when fixation details are missing?
Target-specific fixation sensitivity is unknown from the supplied evidence, and the catalog tissue caption does not report its fixative (datasheet A05324). Record the specimen’s actual fixative, fixation duration, processing history and section age before comparing staining runs (standard IHC practice). On adjacent sections, keep EDTA retrieval at pH 8.0 and the primary concentration at 2 μg/ml constant while investigating documented processing differences (datasheet A05324; standard IHC practice). Use preserved morphology and a no-primary control to judge any apparent gain, since damaged tissue or increased nonspecific DAB can imitate improved antigen detection (standard IHC practice).
What CKAP5 staining pattern should I expect in tissue IHC?
Expect a broadly cytoplasmic tissue pattern: the HPA describes ubiquitous cytoplasmic expression with supported, medium consistency between staining and RNA data (HPA tissue IHC). CKAP5 also associates with centrosomes and kinetochores, and with spindle poles and microtubules during mitosis (UniProt Q14008). Inspect mitotic cells at high magnification before calling a focal structure specific, because chromogenic signal and section geometry can limit resolution of these structures (standard IHC practice). A predominantly crisp nuclear or cell-surface pattern warrants review of morphology and controls rather than immediate assignment to CKAP5, whose annotated locations are cytoskeletal and mitotic (UniProt Q14008; standard IHC practice).
Can this IHC stain distinguish CKAP5 isoforms or modified epitopes?
CKAP5 has 3 annotated isoforms, but the supplied catalog caption does not identify the antibody’s epitope or establish isoform selectivity (UniProt Q14008; datasheet A05324). Its annotated modified residues include acetyllysine at position 48 and phosphoserines at 816, 845, 1469 and 1861 (UniProt Q14008). Do not interpret a change in chromogenic intensity as an isoform shift or phosphorylation change without epitope information and an independently validated, modification-specific assay (standard IHC practice). When comparing specimens, keep retrieval, antibody concentration, incubation and development conditions matched so technical variation is less likely to masquerade as an epitope-specific difference (standard IHC practice).
How can I relate CKAP5 tissue IHC findings to an IF multiplex experiment?
For a separate IF experiment, pair CKAP5 with a validated marker for the cell type under study and use the marker to identify cells before comparing CKAP5 localisation (standard IF practice). Choose fluorophores after inspecting unstained tissue autofluorescence, and include single-label controls to assess bleed-through in the multiplex images (standard IF practice). CKAP5 lacks an annotated transmembrane segment and has cytoskeletal, centrosomal and mitotic locations, so select permeabilisation appropriate for intracellular epitopes and check that it preserves those structures (UniProt Q14008; standard IF practice). The catalog’s 2 μg/ml overnight incubation and EDTA pH 8.0 retrieval come from paraffin-section chromogenic IHC; optimise IF conditions separately (datasheet A05324; standard IF practice).
How do I reduce diffuse DAB or tissue-associated background?
First compare the stained section with a no-primary control and examine whether DAB follows tissue edges, damaged areas or intact cell bodies (standard IHC practice). The catalog tissue example used 10% goat serum, 2 μg/ml primary antibody overnight at 4°C, and a peroxidase-conjugated secondary for 30 minutes at 37°C (datasheet A05324). A peroxidase block, appropriate washing and controlled DAB development are general chromogenic workflow steps to assess background, rather than CKAP5-specific findings (standard IHC practice). If diffuse staining persists, adjust one variable at a time and retain sections showing intact morphology for comparison (standard IHC practice).
What is a defensible way to score CKAP5 chromogenic staining? ⚠ ANSWER MARKED FOR VERIFICATION
Define the compartment and cell population before scoring, using cytoplasmic staining as the principal tissue readout (HPA tissue IHC; standard IHC practice). For intact cells, record percentage positive and intensity by a prespecified rule, then calculate an H-score if graded intensity is reproducible; report the scoring bins and threshold (standard IHC practice). If counting focal mitotic profiles, report positive cells per mm² of evaluable tissue and the number of mitotic cells assessed, since CKAP5 occupies spindle structures during mitosis (UniProt Q14008; standard IHC practice). Normalise comparisons to the same eligible cell population or tissue area and use matched staining runs and exposure-independent image settings (standard IHC practice).
When is CKAP5 staining convincing rather than an artefact?
A convincing result places signal in intact cell bodies with a mainly cytoplasmic pattern, with any finer mitotic localisation judged against cell morphology (HPA tissue IHC; UniProt Q14008; standard IHC practice). HPA reports high staining in adipocytes, cerebral cortical neuronal cells and colon endothelial cells, so identify the actual stained cell population before generalising across tissue compartments (HPA tissue IHC). Treat isolated nuclear or sharp membrane outlines cautiously because they do not match CKAP5’s principal annotated cytoskeletal locations (UniProt Q14008; standard IHC practice). Edge staining, necrotic areas and signal persisting in a no-primary control should prompt review for section artefact or endogenous peroxidase before a positive call (standard IHC practice).
Boster reagents
Best CKAP5 / Cytoskeleton-associated protein 5 IHC Antibodies
A05324 and M05324 have paraffin-section IHC images from human tissues and rat brain; A05324 also has mouse brain IHC (catalog IHC captions). Their IF/ICC images show A431 and HeLa cells, respectively (catalog IF captions).
Real IHC data
Anti-ch TOG/CKAP5 Antibody ®
Cat # A05324
Real IHC data
Anti-ch TOG/CKAP5 Antibody ® (monoclonal, 3C13)
Cat # M05324
A05324 has IHC images from human liver and prostate cancer tissue and mouse and rat brain, plus an IF/ICC image from A431 cells (A05324 image captions). M05324 has IHC images from human liver, rectal and renal cancer tissue and rat brain, plus an IF/ICC image from HeLa cells (M05324 image captions).
Which to pick: For paraffin-section IHC across human, mouse and rat samples, choose A05324: its own IHC captions show all three species, while M05324’s show human and rat tissue (catalog IHC captions). For IF/ICC, A05324 is a rabbit antibody imaged in A431 cells, while M05324 is a mouse monoclonal antibody, clone 3C13, imaged in HeLa cells (catalog host, clone and IF captions). Both IHC captions describe paraffin sections, but neither reports the fixative (catalog IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q14008 (CKAP5_HUMAN, Cytoskeleton-associated protein 5).
- Human Protein Atlas. CKAP5 tissue IHC expression (reliability: Supported).
- Human Protein Atlas. CKAP5 subcellular location (ICC-IF): Mainly localized to the centrosome and basal body. In addition localized to the nucleoli, nucleoli rim, plasma membrane, mitotic spindle and primary cilium..
- Human Protein Atlas. CKAP5 antibody validation summary (2 antibodies).
- CKAP5 deficiency induces premature ovarian insufficiency. EBioMedicine 2025 — PMC12032925.
- Therapeutic gene silencing of CKAP5 leads to lethality in genetically unstable cancer cells. Science advances 2023 — PMC10075965.
- PubMed PMID:8536682 — UniProt-cited evidence.
- PubMed PMID:7788527 — UniProt-cited evidence.
- PubMed PMID:16554811 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for CKAP5 (Cytoskeleton-associated protein 5). Expected localisation Cytoplasmic staining in tissue IHC (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet A05324). Validated IHC antibody: A05324 at 2-5μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adipose tissue (Adipocytes); negative None in HPA (detected in all 45 tissues); use no-primary + isotype controls. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human liver cancer tissue; fixative not specified (datasheet A05324); 2. Fixation: Image fixative and duration unreported (datasheet A05324); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet A05324); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A05324); 8. Primary antibody: Rabbit anti-CKAP5, 2-5μg/ml (datasheet A05324); 9. Primary incubation: Overnight at 4 °C (datasheet A05324). Watch for: Fixation: Keep paraffin-section fixation consistent (standard IHC practice; not target-specific); Caveat: Mitotic spindle localization may alter the pattern (UniProt); Isoform / epitope: 3 isoforms; verify epitope coverage for each (UniProt); Regulation: Elevated in hepatomas and colonic tumors (UniProt). Evidence: UniProt Q14008, HPA tissue IHC · Supported, HPA ICC-IF · Mainly localized to the centrosome and basal body. In addition…, Datasheet A05324, PMC10075965. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/CKAP5/immunohistochemistry-ihc-guide.