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- Table of Contents
CLDN11 / Claudin-11 · Western blot design guide
Design a Western Blot for CLDN11
Source-linked CLDN11 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CLDN11 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CLDN11 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~22 kDa | |
| Observed band | ~22 kDa | |
| Gel | 5–20% (catalog A08381-1) | |
| Positive control | Duodenum (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked CLDN11 Western Blot Protocol Options
The A08381-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human HUVEC (catalog A08381-1) |
| Gel % | 5–20% (catalog A08381-1) |
| Load | 30 ug; reducing conditions (catalog A08381-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A08381-1) |
| Membrane | nitrocellulose membrane (catalog A08381-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A08381-1) |
| Primary antibody | A08381-1 · 0.5 μg/mL (catalog A08381-1) |
| Primary incubation | overnight at 4°C (catalog A08381-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A08381-1) |
| Secondary incubation | 1.5 hour at RT (catalog A08381-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A08381-1) |
| Detection | ECL (catalog A08381-1) |
Section 2
What Is the Expected CLDN11 Western Blot Band Size?
CLDN11 is predicted at 22 kDa, and antibody QC reports a ~22 kDa band; its documented features do not establish another migration pattern.
| Band at ~22 kDa | Matches the reported CLDN11 band and UniProt predicted mass |
| Faint ~22 kDa band in soluble extract | CLDN11 is a multi-pass membrane protein that may remain poorly extracted |
| Stronger ~22 kDa band in membrane-enriched extract | Consistent with CLDN11 cell-membrane localization |
| Near-22 kDa doublet | Could reflect phosphorylation at Ser197 or Ser198; band identity needs confirmation |
💡Expected CLDN11 appearanceUniProt predicts 22 kDa, and antibody QC reports a ~22 kDa band; confirm identity with an appropriate positive control or CLDN11 depletion.
| UniProt predicted mass | Places full-length CLDN11 near 22 kDa, matching the reported band |
| Phosphoserine at Ser197 | May affect migration, but no visible shift is established |
| Phosphoserine at Ser198 | May affect migration, but no visible shift is established |
| Multi-pass membrane localization | Can affect protein recovery; a size shift is not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated CLDN11 may be poorly extracted | Check the membrane fraction and extraction conditions |
| Band higher than expected | Incomplete solubilization of this multi-pass membrane protein is possible | Optimize denaturation and confirm the band by CLDN11 depletion |
| Broad smear instead of sharp band | Incomplete membrane-protein solubilization is possible | Optimize detergent extraction and sample preparation |
| Multiple bands | Phosphorylation at Ser197 or Ser198 is possible, but distinct bands are unproven | Compare phosphatase-treated samples and confirm identity by CLDN11 depletion |
| Weak or no signal | CLDN11 may be poorly recovered from the membrane fraction | Check membrane enrichment and include a CLDN11-positive control |
Experiment evidence
Western blot images showing CLDN11 band
A08381-1 · CLDN11
M08381 · CLDN11
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CLDN11 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CLDN11 in Western blot, you can use duodenum tissue.
Positive control: Duodenum (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: As a multi-pass membrane protein, CLDN11 may be easier to detect in membrane-enriched lysate.
HPA tissue expression evidence for CLDN11
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Duodenum | paneth cells | High | Protein (IHC) | HPA → |
| Skin | fibrohistiocytic cells | High | Protein (IHC) | HPA → |
| Small intestine | paneth cells | High | Protein (IHC) | HPA → |
| Testis | sertoli cells | High | Protein (IHC) | HPA → |
| Caudate | glial cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | endocrine cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CLDN11 Western Blot Tips
Deeper troubleshooting and optimisation questions for CLDN11, answered from its protein features.
How should CLDN11 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a second CLDN11 band be a listed isoform?
Isoforms · The supplied UniProt record lists one isoform and no alternative sequence. It therefore provides no basis to assign another band to a documented CLDN11 isoform.
Which CLDN11 phosphorylation sites matter when interpreting a band shift?
PTM · UniProt lists phosphoserines at positions 197 and 198 in the 207-residue sequence. These are UniProt coordinates; paper or antibody numbering may differ. Their annotation alone cannot explain an observed mass difference.
Does this guide establish induction of CLDN11?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for CLDN11 Western blot?
Transfer · CLDN11 is a ~22 kDa multi-pass membrane protein. Choose transfer conditions that retain proteins near 22 kDa, and verify transfer at that size. The supplied features do not establish one optimal transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A08381-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should CLDN11 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should CLDN11 appear above its predicted 22 kDa mass?
Interpretation · The supplied apparent band is ~22 kDa, matching the predicted mass. Phosphorylation is annotated, but its presence alone does not establish a visible shift.
CLDN11 localizes to the cell membrane and tight junctions. Use the same sample preparation and fraction across samples when comparing its ~22 kDa band; differences in membrane recovery can affect the comparison.
The record lists interactions with OCLN and, by similarity, TSPAN3. Those interactions do not establish that a higher Western-blot band is a CLDN11 oligomer. Confirm the band’s identity before assigning it to an interaction or modification.
CLDN11 localizes to the cell membrane and tight junctions. Use the same sample preparation and fraction across samples when comparing its ~22 kDa band; differences in membrane recovery can affect the comparison.
The record lists interactions with OCLN and, by similarity, TSPAN3. Those interactions do not establish that a higher Western-blot band is a CLDN11 oligomer. Confirm the band’s identity before assigning it to an interaction or modification.
Boster reagents
CLDN11 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-CLDN11 Antibody Picoband®
Cat # A08381-1
Real WB data
Anti-Claudin 11 CLDN11 Monoclonal Antibody
Cat # M08381
Two the supplier anti-CLDN11 antibodies have WB images. A08381-1 shows an approximately 22 kDa band in human HUVEC lysate at 0.5 μg/mL. M08381 shows a blot using SH-SY5Y lysate, but its supplied caption gives no band size or assay conditions.
Which to pick: Choose A08381-1 for the documented human HUVEC WB example. Consider M08381 if you need catalogued mouse or rat reactivity; its supplied WB example uses SH-SY5Y lysate and does not document mouse or rat samples.
Source: BosterBio CLDN11 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O75508.
- Human Protein Atlas. CLDN11 tissue expression.
- PMC5938033 — target-verified WB comparison
- PMC2776495 — target-verified WB comparison
- PMC10993429 — target-verified WB comparison