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- Table of Contents
COIL / Coilin · Western blot design guide
Design a Western Blot for COIL
Real validated COIL Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-COIL WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
COIL Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~62.6 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Testis (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Ubl conjugation | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated COIL Western Blot Protocols
The M02784 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Jurkat cell lysate (catalog M02784) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M02784; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected COIL Western Blot Band Size?
COIL has a predicted 62.6 kDa backbone; its documented phosphorylation may cause modest variation in apparent band position.
| Band near 62.6 kDa | Coilin near its predicted mass |
| Closely spaced bands near 62.6 kDa | Coilin with differing phosphorylation states |
| Slightly shifted band near 62.6 kDa | Phosphorylation may alter migration |
| Weak band in a nuclear fraction | Variable recovery of nuclear Coilin |
💡Expected COIL appearanceExpect a Coilin band near its predicted 62.6 kDa, with possible modest mobility variation associated with its documented phosphorylation sites.
| Predicted Coilin mass | Places the protein backbone at 62.6 kDa |
| Phosphoserine 105 | May contribute to modest mobility variation |
| Phosphothreonine 122 | May contribute to modest mobility variation |
| Phosphoserine 184 | May contribute to modest mobility variation |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear Coilin may be poorly recovered | Check nuclear extraction and loading |
| Band higher than expected | Phosphorylation may alter migration | Compare migration before and after phosphatase treatment |
| Band lower than expected | Possible protein cleavage during preparation | Prepare fresh lysate with protease inhibitors |
| Multiple bands | Different phosphorylation states may migrate differently | Compare bands before and after phosphatase treatment |
| Weak or no signal | Low recovery of nuclear Coilin | Check a nuclear fraction and loading |
Experiment evidence
Western blot images showing COIL band
M02784 · COIL
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for COIL Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for COIL in Western blot, you can use testis lysate, the highest-scoring HPA positive tissue.
Positive control: Testis (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA lists a tissue with no detected COIL, though a nuclear protein may require adequate nuclear protein extraction for a strong positive signal.
HPA tissue expression evidence for COIL
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | pachytene spermatocytes | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells - nucleus | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Not detected | Protein (IHC) | HPA → |
| Lung | alveolar cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced COIL Western Blot Tips
Deeper troubleshooting and optimisation questions for COIL, answered from its protein features.
Where should the main COIL band appear?
Band shift · COIL has a predicted mass of 62.6 kDa. Use that as a starting point; no empirical apparent band position is supplied.
Do COIL isoforms explain multiple bands?
Isoforms · Only one isoform is listed. Do not assign additional bands to annotated isoforms on this evidence alone.
Could modifications shift COIL from its predicted mass?
PTM · COIL has 16 annotated modified residues and is identified as a phosphoprotein and a protein subject to ubiquitin-like conjugation. These features can affect migration, but the supplied data do not establish a specific shift.
Does this guide establish induction of COIL?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for COIL?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M02784 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Can COIL signal be used to compare samples quantitatively?
Quantitation · COIL is nuclear and has annotated modifications. Compare like sample fractions and use a consistent reference for loading; a change in one band alone does not establish a change in total COIL if modified forms are present.
Which sample fraction is appropriate for detecting COIL?
Interpretation · COIL is located in the nucleus and Cajal bodies. A nuclear fraction is therefore a relevant sample when assessing its detection.
Compare them with the 62.6 kDa predicted mass, while considering COIL's annotated modified residues and ubiquitin-like conjugation. The supplied features do not identify the sizes or causes of additional bands; confirm their identity independently.
Compare them with the 62.6 kDa predicted mass, while considering COIL's annotated modified residues and ubiquitin-like conjugation. The supplied features do not identify the sizes or causes of additional bands; confirm their identity independently.
Boster reagents
COIL Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Coilin Rabbit Monoclonal Antibody
Cat # M02784
The catalog reports one anti-COIL antibody, M02784, with a Western blot image showing Coilin expression in Jurkat cell lysate. The supplied evidence does not establish performance in other samples.
Which to pick: M02784 is the only listed option and has a Western blot image from Jurkat cell lysate. If your samples differ, confirm suitability for your intended application.
Source: BosterBio COIL gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P38432.
- Human Protein Atlas. COIL tissue expression.
- PMC12221373 — target-verified WB comparison