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CORO1A / Coronin-1A · Western blot design guide
Design a Western Blot for CORO1A
Source-linked CORO1A Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CORO1A WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CORO1A Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~51 kDa | |
| Observed band | ~57 kDa | |
| Gel | 5–20% (catalog A04245-2) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked CORO1A Western Blot Protocol Options
The A04245-2 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Jurkat, rat spleen, rat thymus, mouse spleen, mouse thymus (catalog A04245-2) |
| Gel % | 5–20% (catalog A04245-2) |
| Load | 30ug; reducing conditions (catalog A04245-2) |
| Transfer | a Nitrocellulose membrane at 150mA for 50-90 minutes (catalog A04245-2) |
| Membrane | Nitrocellulose membrane (catalog A04245-2) |
| Blocking | 5% Non-fat Milk/ TBS for 1.5 hour at RT (catalog A04245-2) |
| Primary antibody | A04245-2 · 0.5 μg/mL (catalog A04245-2) |
| Primary incubation | overnight at 4°C (catalog A04245-2) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A04245-2) |
| Secondary incubation | 1.5 hour at RT (catalog A04245-2) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A04245-2) |
| Detection | ECL (catalog A04245-2) |
Section 2
What Is the Expected CORO1A Western Blot Band Size?
CORO1A is predicted at 51 kDa and observed near 57 kDa on antibody QC blots; the cause of the difference is not established.
| Band near 57 kDa | Matches the reported CORO1A band; confirm identity with controls |
| Band near 51 kDa | Near the UniProt predicted mass; identity needs confirmation |
| Close doublet near the main band | Could reflect phosphorylation at Ser2, Thr412, or Ser422; a shift is unproven |
| Weak band in a soluble fraction | CORO1A may partition with the cytoskeleton or phagosome membranes |
💡Expected CORO1A appearanceUniProt predicts 51 kDa, while antibody QC reports a band near 57 kDa in reducing lysates; the cause of the difference is unestablished, so confirm identity with appropriate controls.
| UniProt predicted mass | 51 kDa by sequence; antibody QC reports an apparent band near 57 kDa |
| Ser2, Thr412, and Ser422 phosphorylation | Could affect migration, but no visible shift is established |
| Ser2 N-acetylation | Present as an annotated modification; no band shift is established |
| Lys449 N6-acetylation | Present as an annotated modification; no band shift is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | CORO1A may partition with cytoskeletal or membrane-associated material | Check the insoluble fraction and a positive-control lysate |
| Band higher than expected | The reported 57 kDa migration exceeds the 51 kDa sequence prediction; its cause is unknown | Compare with the reported 57 kDa band and confirm identity by CORO1A depletion |
| Band lower than expected | A smaller band is not explained by an annotated signal peptide or propeptide | Compare with a positive control and test whether CORO1A depletion removes the band |
| Multiple bands | Phosphorylation states are possible, but distinct band migration is unproven | Compare phosphatase-treated and untreated samples and verify band identity |
| Weak or no signal | Cytoskeletal or membrane association may reduce recovery in the tested fraction | Check fraction recovery and include a positive-control lysate |
| Fragments below expected size | The supplied features do not establish a cleavage product | Check sample integrity and test fragment identity with CORO1A depletion |
Experiment evidence
Western blot images showing CORO1A band
A04245-2 · CORO1A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CORO1A Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CORO1A in Western blot, you can use appendix tissue, which HPA rates High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Cytoplasmic CORO1A is suitable for tissue lysates, and HPA identifies a not-detected tissue for a negative control.
HPA tissue expression evidence for CORO1A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | non-germinal center cells | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Cerebellum | granular cells - cytoplasm/membrane | High | Protein (IHC) | HPA → |
| Colon | mucosal lymphoid cells | High | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Cervix | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CORO1A Western Blot Tips
Deeper troubleshooting and optimisation questions for CORO1A, answered from its protein features.
How should CORO1A band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a CORO1A isoform explain an additional band?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no isoform-based explanation for an additional band; check band identity before assigning one to CORO1A.
Which CORO1A modifications should guide band interpretation?
PTM · Using the supplied UniProt coordinates, Ser2 is listed as N-acetylserine and as a PKC phosphorylation site; Thr412 is phosphorylated by PKC; Ser422 is phosphorylated; and Lys449 is acetylated. These annotations do not establish that any modification resolves as a separate band. Check numbering conventions when comparing antibody or paper descriptions.
Does this guide establish induction of CORO1A?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CORO1A?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04245-2 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should CORO1A Western blot signal be quantified?
Quantitation · Quantify the confirmed CORO1A band under consistent loading, extraction and fractionation conditions. Because CORO1A associates with cytoskeletal and phagosomal compartments, compare like fractions and distinguish changes in a fraction from changes in total cellular CORO1A.
Why might CORO1A appear near 57 kDa instead of 51 kDa?
Interpretation · Its predicted mass is 51 kDa, while the reported apparent band is about 57 kDa. UniProt lists modifications, but their presence alone does not establish a visible shift or explain this difference. Use the observed band as a reference and confirm its identity independently.
PKC is listed for phosphorylation at Ser2 and Thr412, using the supplied UniProt coordinates. If comparing conditions for phosphorylation, assess those sites with a suitable site-specific measurement alongside total CORO1A. The record does not identify a stimulus or predict a band shift.
The record places CORO1A at the cytoskeleton, cell cortex and phagosome membrane. In mycobacteria-infected macrophages, it describes progressive relocalization and retention. Keep extraction and fractionation consistent across samples; a change in one fraction may reflect redistribution rather than a change in total abundance.
The supplied record lists one isoform, no alternative sequence, and no signal peptide, propeptide or glycosylation sites. It does not identify a source for extra bands. Check band identity and sample preparation before attributing them to a CORO1A isoform or modification.
PKC is listed for phosphorylation at Ser2 and Thr412, using the supplied UniProt coordinates. If comparing conditions for phosphorylation, assess those sites with a suitable site-specific measurement alongside total CORO1A. The record does not identify a stimulus or predict a band shift.
The record places CORO1A at the cytoskeleton, cell cortex and phagosome membrane. In mycobacteria-infected macrophages, it describes progressive relocalization and retention. Keep extraction and fractionation consistent across samples; a change in one fraction may reflect redistribution rather than a change in total abundance.
The supplied record lists one isoform, no alternative sequence, and no signal peptide, propeptide or glycosylation sites. It does not identify a source for extra bands. Check band identity and sample preparation before attributing them to a CORO1A isoform or modification.
Boster reagents
CORO1A Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Coronin 1a/TACO/CORO1A Antibody Picoband®
Cat # A04245-2
The catalog reports A04245-2, a rabbit polyclonal anti-CORO1A antibody with a Western blot image showing an approximately 57 kDa band in human Jurkat cells and rat and mouse spleen and thymus lysates. The supplied evidence is limited to this product example.
Which to pick: A04245-2 is the only listed option. Its WB example uses human Jurkat cells and rat and mouse spleen and thymus lysates under reducing conditions, with antibody at 0.5 μg/mL. Match your planned sample and conditions to that example.
Source: BosterBio CORO1A gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P31146.
- Human Protein Atlas. CORO1A tissue expression.
- PMC7391189 — target-verified WB comparison
- PMC13293819 — target-verified WB comparison
- PMC11760921 — target-verified WB comparison