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- Table of Contents
COX10 / Protoheme IX farnesyltransferase, mitochondrial · Western blot design guide
Design a Western Blot for COX10
Source-linked COX10 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-COX10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
COX10 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~48.9 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Epididymis (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | Membrane extraction controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked COX10 Western Blot Protocol Options
The A05710 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | mouse-kidney mouse-brain Hela KB 293T lysis (catalog A05710) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A05710; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected COX10 Western Blot Band Size?
COX10 is predicted at 48.9 kDa; isoforms may affect migration, but no empirical band size or distinct isoform bands are demonstrated.
| Single band near 48.9 kDa | Consistent with the predicted COX10 mass; identity still requires controls. |
| Single band away from 48.9 kDa | An isoform is possible, but its migration is unknown. |
| Several discrete bands | Isoforms 1 and 2 are possible contributors; band identities are unconfirmed. |
| Band enriched in a mitochondrial membrane fraction | Consistent with COX10's mitochondrial membrane location. |
💡Expected COX10 appearanceCOX10 has a predicted mass of 48.9 kDa, but no empirical band size is supplied; use band-identity controls because the migration of isoforms 1 and 2 is unknown.
| Predicted COX10 mass | Provides a 48.9 kDa reference, not a measured band position. |
| Isoform 1 | Its apparent size relative to isoform 2 is not supplied. |
| Isoform 2 | Its apparent size relative to isoform 1 is not supplied. |
| Alternative splicing of isoforms 1 and 2 | May affect band size; distinct migration has not been demonstrated. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Mitochondrial membrane COX10 may be poorly recovered. | Check mitochondrial membrane recovery and a positive control. |
| Band higher than expected | Band identity or isoform migration is unresolved. | Compare a positive control and an independent COX10 antibody. |
| Band lower than expected | An isoform or an unrelated band is possible; isoform sizes are unknown. | Use an independent COX10 antibody or genetic depletion to check identity. |
| Multiple bands | Isoforms 1 and 2 are possible, but their separation is unverified. | Check which bands respond to COX10 depletion. |
| Weak or no signal | Recovery of this multi-pass mitochondrial membrane protein may be low. | Check membrane-protein extraction and loading with a mitochondrial marker. |
Experiment evidence
Western blot images showing COX10 band
A05710 · COX10
A05710 · COX10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for COX10 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for COX10 in Western blot, you can use epididymis tissue, which has high HPA expression.
Positive control: Epididymis (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside.
⚠️Feasibility: No tissue is reported as not detected, so use siRNA knockdown or a KO line for a negative control.
HPA tissue expression evidence for COX10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Epididymis | glandular cells | High | Protein (IHC) | HPA → |
| Hippocampus | neuronal cells | High | Protein (IHC) | HPA → |
| Kidney | bowman's capsule | High | Protein (IHC) | HPA → |
| Lung | alveolar cells type I | High | Protein (IHC) | HPA → |
| Placenta | cytotrophoblasts | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Smooth muscle | smooth muscle cells | Low | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced COX10 Western Blot Tips
Deeper troubleshooting and optimisation questions for COX10, answered from its protein features.
How should COX10 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could COX10 isoforms produce different bands?
Isoforms · Two isoforms are listed. In isoform 2, residues 1–15 are replaced and residues 16–207 are missing, using canonical UniProt coordinates. A size difference is plausible, but no apparent band size is supplied for either isoform. Check whether the antibody's target sequence is retained before assigning a band.
Is a COX10 band shift explained by an annotated modification?
PTM · No modified residues, glycosylation sites, or disulfides are listed. The supplied features therefore do not support assigning a shift to one of these modifications. They also do not rule out unannotated changes; confirm a band’s identity before explaining its migration.
Does this guide establish induction of COX10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for COX10 Western blot?
Transfer · COX10 is a 443-residue, multi-pass mitochondrial membrane protein. The supplied features do not establish a preferred transfer method. Check transfer efficiency around the predicted 48.9 kDa region and optimize the method empirically before comparing samples.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A05710 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify COX10 bands across samples?
Quantitation · Keep membrane-protein extraction, loading, and transfer consistent across samples. Quantify the same identified band each time. Because two isoforms are listed and their apparent band positions are not supplied, establish band identity before combining signals or comparing isoform-specific amounts.
Should COX10 run at exactly 48.9 kDa?
Interpretation · 48.9 kDa is the predicted mass, and no observed band size is supplied. Use it as a reference, not an exact migration target. COX10 is a multi-pass mitochondrial membrane protein, but that feature alone does not explain any difference between predicted and apparent mass.
Compare it with the predicted 48.9 kDa mass, then consider the two listed isoforms and whether the antibody's target sequence is present in each. Isoform 2 replaces canonical residues 1–15 and lacks residues 16–207. Neither these sequence features nor COX10's membrane location alone identifies an unexpected band; confirm its identity experimentally.
Compare it with the predicted 48.9 kDa mass, then consider the two listed isoforms and whether the antibody's target sequence is present in each. Isoform 2 replaces canonical residues 1–15 and lacks residues 16–207. Neither these sequence features nor COX10's membrane location alone identifies an unexpected band; confirm its identity experimentally.
Boster reagents
COX10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-COX10 Antibody
Cat # A05710
A05710 is an anti-COX10 antibody listed for human and mouse. Its Western blot image shows mouse kidney, mouse brain, HeLa, KB, and 293T lysates at a 1:1000 antibody dilution. No publication evidence is supplied.
Which to pick: A05710 is the only listed COX10 antibody. It has a Western blot image covering the named mouse tissues and cell lines; use the reported 1:1000 dilution as a starting point for those sample types.
Source: BosterBio COX10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q12887.
- Human Protein Atlas. COX10 tissue expression.
- PMC8734181 — target-verified WB comparison
- PMC11112789 — target-verified WB comparison