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- Table of Contents
CRK · Western blot design guide
CRK Western Blot Planning Guide
Plan a CRK Western blot around the catalog-observed 33.8 kDa band, image-backed PB9101 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CRK Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 33.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Salivary gland | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 33.8 kDa |
| ⓘ | Localization | Cytoplasm / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for CRK Western blot
🧪Use Salivary gland as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Salivary gland (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CRK
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Salivary gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Small intestine | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Liver | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 1
What Is the Expected CRK Western Blot Band Size?
Use the product-observed 33.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 33.8 kDa | Matches the authoritative product WB observation. |
| 33.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CRK appearancePlan around 33.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 33.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 33.8 kDa from UniProt P46108; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9101. |
| Situation | Likely cause | Next action |
|---|---|---|
| 33.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CRK band
PB9101 · CRKObserved 33.8 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated CRK Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Duodenum |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9101 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced CRK Western Blot Tips
Deeper troubleshooting and optimisation questions for CRK, answered from its protein features.
Which band should guide the blot?
Use 33.8 kDa, the observation attached to the authoritative PB9101 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 33.8 kDa expectation.
Which positive control should I start with?
Start with Salivary gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CRK Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9101 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC3938434, PMC2175095.
Boster reagents
CRK Western Blot Reagents
Human/Mouse/Rat-reactive CRK Western blot reagents with authoritative product imagery.
Real WB data
Anti-Crk p38 Antibody Picoband®
Cat # PB9101
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P46108
- Human Protein Atlas — CRK
- PB9101 product record
- PMC3938434 — Combining the ABL1 kinase inhibitor ponatinib and the histone deacetylase inhibitor vorinostat: a potential treatment for BCR-ABL-positive leukemia.
- PMC2175095 — Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the extracellular matrix.