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- Table of Contents
1 Citations 15 Q&As
Facts about Epithelial discoidin domain-containing receptor 1.
Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal ear morphology and normal hearing (By similarity).
Human | |
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Gene Name: | DDR1 |
Uniprot: | Q08345 |
Entrez: | 780 |
Belongs to: |
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protein kinase superfamily |
CAK; CD167 antigen-like family member A; CD167a antigen; CD167a; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EC 2.7.10; EC 2.7.10.1; EDDR1; ENTRK4; Epithelial discoidin domain receptor 1; HGK2; MCK10; MCK-10; NTRK4; Protein-tyrosine kinase 3A; PTK3A protein tyrosine kinase 3A; PTK3A; receptor, type 4; RTK6; TRK E; TrkE
Mass (kDA):
101.128 kDA
Human | |
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Location: | 6p21.33 |
Sequence: | 6; NC_000006.12 (30880958..30900156) |
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
Boster Bio Anti–MCK10/DDR1 marker is an extremely high-quality DDR1 anti-body. But, you may be thinking about what this antibody is about and how it could enhance your research. We will talk about the advantages of this antibody in your research and the other protein that can be analyze using an ELISA.
The Boster Bio Anti-MCK10/DR1 Marker an antibody humanized that recognizes the extracellular domain of the human brain tumor protein DDR1. This marker is used to investigate the role of DDR1 and its impact on the development of astrocytoma. The antibody can be used to identify and treat brain tumors. It is being evaluated both in the laboratory and in the clinic to determine potential treatments.
The anti-DDR1 marker can be made by using Biacore SPR technology, which evaluates the binding affinities of DDR1 antibodies. A first molecule is coupled to an CM-5 sensor chip from Dextran that captures the antibody. The antigen is then injected at a specified flow rate, and then the buffer is subsequently added. The antigen and buffer are then introduced in a sequence into the sample. The BIA evaluation software measures the dissociation and association rates.
Steven Boster developed his first product in 1993, earning him the moniker "he who converts science into the lavatory". His success as an innovator in the field of antibodies led to the development of hundreds of primary antibodies, ELISA kits, and IHC products. He was able to create the largest catalog antibody company in China in the latter part of the 90s. Boster Bio developed high-sensitivity ELISA kits using trade secrets that analyze the level of cellular activity and molecular activity from just one sample.
ELISA is a method of measuring the concentrations of proteins and other compounds in a solution by using an enzyme-linked immunosorbent assay. In the traditional method, chromogenic substrates and chromogenic reporters are employed. Nowadays, newer ELISA-like techniques use fluorescent, electrochemiluminescent, or quantitative PCR reporters. The newer methods are often called ELISA-like since they offer numerous advantages over traditional tests, including higher levels of sensitivities as well as multiplexing. Additionally they are not enzymatic and do not require enzyme linkage, making them suitable for other applications , too.
ELISA allows qualitative analysis of levels of protein in addition to quantitative methods. The qualitative information is usually shown as a graph of optical density versus log concentration of the sample. Standard curves can be generated by analyzing concentrations of known analytes. These curves can be used to calculate the unknown analyte concentration. It is recommended that ELISA plates should be kept at ambient temperature and free of drafts to prevent contamination.
Sandwich ELISA is a multimeric protein quantification method that was developed specifically for food samples. It is especially beneficial for analyzing recombinant c4H in crude E.coli extracts. Sandwich ELISA detects all holoenzymes and does not suffer from background expression. This method is perfect for quantitatively measuring proteins. It is also widely employed in proteomic research as well as food samples analysis.
In addition to the routine ELISA C-P4H tetramer was developed to enhance its sensitivity. The IPTG concentration of 10 was used to determine the response. It was measured at 5.5, 12.5, 24.5 and 24.5 hours after induction. The lowest yield was observed in experiments that utilized early induction. However, this yield increased over time. After 12.5 hours of induction, the highest yields were recorded.
Sandwich ELISA is a different method to detect proteins. This method involves a combination of two antibodies that one of which binds to a target protein and the other one to a chemical substrate, thereby creating a colorimetric signal. ELISA plate readers can also detect this signal. Sandwich ELISA can be used to determine the concentration of proteins in tissues and in vitro. A sandwich ELISA is a method to test a variety of proteins in your sample.
ELISA can also serve other ligand binding assays. Its primary reagent immobilized on a solid phase is then connected to a secondary antibody. The secondary antibody is then able to bind the ligand , while the enzyme identifies the analyte. The ligand is still bound to the analyte in a typical ELISA even after repeated washes. However other components that are not specific are washed away from the solid phase.
ELISA employs an antibody that is specifically designed for a particular antigen. The immobilized antibody recognizes and binds to the target protein in the sample. The second antibody binds to another epitope of the same protein. The antibodies react with the second antibody, and the result is a change in color. This is a straightforward accurate, reliable, and sensitive method of detecting proteins. ELISA can give quantitative information about other proteins when used properly.
PMID: 8226977 by di Marco E., et al. Molecular cloning of trkE, a novel trk-related putative tyrosine kinase receptor isolated from normal human keratinocytes and widely expressed by normal human tissues.
PMID: 8390675 by Johnson J.D., et al. A receptor tyrosine kinase found in breast carcinoma cells has an extracellular discoidin I-like domain.
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