Human Total DDR1 ELISA Kit PicoKine®

Product Name

Human Total DDR1 ELISA Kit PicoKine®

SKU/Catalog Number

EK2383

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Total DDR1 ELISA Kit PicoKine® (96 Tests). Quantitate Human Total DDR1 in cell lysates. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Total DDR1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2383)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Asp21 - Thr416

Capture/Detection Antibodies

polyclonal antibody from goat|polyclonal antibody from goat

Sensitivity

<50 pg/mL

Assay Range

312.5-20,000 pg/mL

Standard Dilution Instructions

See datasheet of EK2383 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2383 is reactive to DDR1 in Human samples

Validated Sample Types

cell lysates

Application Guarantee

EK2383 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DDR1

DDR1 (Discoidin domain receptor family, member 1) also known as NEP, EDDR1, NTRK4, TRKE, DDR, CAK or RTK6, is a human gene. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen.This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain.The DDR1 gene is mapped on 6p21.33. The fibrillar collagens and immobilized collagen activatedDDR1receptor phosphorylation after prolonged treatment. Bhatt et al. (2000)showed thatDdr1was highly expressed in the cerebellum of developing and adult mouse brain, and that bothDdr1and collagen IV were highly expressed in the pial layer of the cerebellar cortex.Cocultures of collagen I- and IV-expressing mouse pial cells withDdr1-expressing granule cells resulted in granule cell neurite extension. Inhibition of collagen-Ddr1signaling reduced granule cell neurite elongation.

Kit Components

Catalog Number	Description	Quantity
EK2383-CAP	Anti-Human DDR1 Pre-coated 96-well strip microplate	1
EK2383-ST	Human DDR1 Standard	2 vials, 20 ng/tube
EK2383-DA	Human DDR1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.022	0.105	0.179	0.327	0.619	1.067	1.748	2.411

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Total DDR1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis