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- Table of Contents
Designing a Western blot for DNAJB1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the DNAJB1 Western Blot GuideFacts about DnaJ homolog subfamily B member 1.
Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery period length of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
| Human | |
|---|---|
| Gene Name: | DNAJB1 |
| Uniprot: | P25685 |
| Entrez: | 3337 |

| Belongs to: |
|---|
| No superfamily |

DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1
Mass (kDA):
38.044 kDA

| Human | |
|---|---|
| Location: | 19p13.12 |
| Sequence: | 19; NC_000019.10 (14514764..14530597, complement) |
Cytoplasm. Nucleus. Nucleus, nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.






PMID: 1754405 by Raabe T., et al. A human homologue of the Escherichia coli DnaJ heat-shock protein.
PMID: 8250930 by Ohtsuka K.; Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ.