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- Table of Contents
Facts about DnaJ homolog subfamily B member 1.
Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery period length of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
Human | |
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Gene Name: | DNAJB1 |
Uniprot: | P25685 |
Entrez: | 3337 |
Belongs to: |
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No superfamily |
DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1
Mass (kDA):
38.044 kDA
Human | |
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Location: | 19p13.12 |
Sequence: | 19; NC_000019.10 (14514764..14530597, complement) |
Cytoplasm. Nucleus. Nucleus, nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Scientists have many options regarding the use of DNAJB1 markers for research. Scientists have several options. They can submit species or applications for credit and get credits. These services are available to scientists around the world. They also offer scientists the chance to submit samples for specific tests for research. What are the best uses possible for DNAJB1 How do they benefit scientists and engineers? Find out more by reading on!
The clades that were derived from the hCOV-19 sequences of genome DNA were analyzed and classified according to gene cluster. The clade with oldest sequences originated in Iran and Turkey, specifically. This clade can help researchers track changes in the virus's genome and the regions infected by it. This clade also includes strains from Turkey and Iran, which could be linked to Middle East outbreaks.
Next-generation sequencing technologies are expected reveal more viruses that don’t fit the virus disease model. SARS-CoV-2 virus is one example. The genome sequence contains critical metadata. This data will aid in developing effective approaches to control COVID-19 infections. Scientists from all over are working together to improve our understanding of the disease.
In Taiwan, the COVID-19 pandemic was documented. While the first wave of cases were from China, the second wave was originated in Europe or the Americas. One isolate had a 382-nucleotide deletion at ORF8 in the first wave. 5 clustered cases also failed to trace imported patients and were classified local transmission. These five cases were each assigned an ORF1abV378I, a different mutation from the ORF8L84S. Further, another mutation was identified in hCOV-19, the ORF1ab-V378I.
GISAID contained 80 sequences at the time that this submission was made. The C.1.2 lineage is growing and has been detected in southern Africa and Botswana. Its incidence has increased significantly over the last three year. Despite there being no confirmed cases, it should be considered a rare disease because of its low awareness.
This discovery is a reflection of the fact that human COV-19 and its mouse cousin are more closely related than any other coronavirus species. It also provides a basis to a more definitive classification for the two human coronaviruses. The World Health Organization has declared this disease a pandemic. If the virus persists in people, it can cause severe illness and the outbreak will continue for a long period.
The hCOV-19 DNA has been grouped into a number of new clades. Based on their geographic origin, three clades were divided into ten subclades. There have been several strains of this virus identified in Australia. Europe and the Middle East. Researchers will be able to identify these new strains using data from impartial metagenomics.
The DNAJB1 biomarker is a novel biomarker to detect pancreatic disease. Knockdown DNAJB1 significantly inhibits pancreatic carcinoma cells' migration, invasion, or proliferation. This finding opens the door for the use DNAJB1 to diagnose pancreatic cancer. The literature has discussed several benefits of DNAJB1 knockdown in pancreatic cancer.
DNAJB1 is a powerful prognostic marker for CCA. It may predict poor outcomes by promoting cancer cell proliferation and inhibiting p53-mediated death. Numerous studies have shown that DNAJB1 expression is higher in CCA cells, tissues, bile samples, and CCA cells. DNAJB1 gene expression was associated to pathological differentiation, vessel invasion, T stage, and lymphoma metastasis. CCA patients with high DNAJB1 expression were also less likely to survive.
DNAJB1 not only detects the alphafetoprotein but also allows physicians to determine if a particular patient has the DNAJB1 mutation. DNAJB1 is excellent at binding to many types of cancer cells, and has anti-tumor activity. Patients with DNAJB1 gene mutations in cancer patients are more likely to be diagnosed early with cancer.
This biomarker can also detect tumor protein D52. DNAJB1 is part of the DnaJ heat shock protein family. Physicians can diagnose cancer more accurately by using DNAJB1. This protein can be used as a marker for CCA. However, there are other biomarkers that can be used to distinguish these diseases.
A study using DNAJB1 to detect CCA found that TPD52 and DNAJB1 proteins were more expressed in cancerous tissues than in healthy tissue. TRF may be less sensitive than DNAJB1/TPD52 biomarkers. These two proteins are often combined, which allows scientists to identify CCA cases better. A few studies have shown that DNAJB1 or TPD52 are better biomarkers for CCA cases than TRF.
4 candidate biomarkers could be validated via RT-qPCR or western blot analysis. The 4 DEPs were tumor protein D52 and heat shock protein family member A1 as well as TACC3 transforming alic coiled-containing proteins. These biomarkers were associated to survival rates and other clinical parameters. However, the strongest candidate is TPD52. It also has a clear connection with DNAJB1.
The DNAJB1 marker is used in many areas of cancer research. This marker is involved with the migration process and interacts to Hsp40/DnaJ proteins, promoting protein transport as well as degradation. DNAJB1 is also known to inhibit the ubiquitination p53 and prevent its degradation by proteasomes. DNAJB1 depletion causes cancer cell growth to increase and promotes activation of the p53 pathway.
The DNAJB1-PRKACA fusion is a diagnostic test for fibrolamellar hepatocellular carcinoma, and five pancreatobiliary neoplasms harbor this mutation. The next step would be to develop a drug to inhibit the activity of protein kinases in pancreatobiliary neoplasms. These are still experimental.
PMID: 1754405 by Raabe T., et al. A human homologue of the Escherichia coli DnaJ heat-shock protein.
PMID: 8250930 by Ohtsuka K.; Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ.