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Product Info Summary
| SKU: | M03100 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9]
SKU/Catalog Number
M03100
Size
100 µl
Description
Boster Bio DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1E9 (formerly 1E9). Catalog# M03100. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03100)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1E9
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
Reactive Species
M03100 is reactive to DNAJB1 in Human, Mouse, Rat
Observed Molecular Weight
37.9 kDa
Calculated molecular weight
38.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03100 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000~2000
IHC: 1:150
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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Western blot analysis of DNAJB1 using anti-DNAJB1 antibody (M03100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DNAJB1 antigen affinity purified monoclonal antibody (Catalog # M03100) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DNAJB1 at approximately 38 kDa. The expected band size for DNAJB1 is at 38 kDa.
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Flow cytometric Analysis of Hela cells
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Western blot analysis of extracts (10ug) from Hela cell line by using anti-DNAJB1 monoclonal antibody (1:200).
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HEK293T cells transfected with either DNAJB1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (M03100)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:100).
Specific Publications For Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (M03100)
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