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- Table of Contents
DSC1 / Desmocollin-1 · Western blot design guide
Design a Western Blot for DSC1
Source-linked DSC1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-DSC1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
DSC1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~100 kDa | |
| Observed band | ~100 kDa | |
| Gel | 8% (catalog M03478) | |
| Positive control | Hair (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Precursor versus mature forms | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked DSC1 Western Blot Protocol Options
The M03478 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human K562, rat lung (catalog M03478) |
| Gel % | 8% (catalog M03478) |
| Load | 30 ug; reducing conditions (catalog M03478) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M03478) |
| Membrane | nitrocellulose membrane (catalog M03478) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M03478) |
| Primary antibody | M03478 · 1:500 (catalog M03478) |
| Primary incubation | overnight at 4°C (catalog M03478) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog M03478) |
| Secondary incubation | 1.5 hour at RT (catalog M03478) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M03478) |
| Detection | ECL (catalog M03478) |
Section 2
What Is the Expected DSC1 Western Blot Band Size?
DSC1 is predicted at 100 kDa and observed at ~100 kDa; its listed processing, glycosylation, and isoforms do not establish additional band positions.
| Band at ~100 kDa | Matches the observed DSC1 band and the predicted 100 kDa mass; confirm identity with an appropriate control |
| Band below ~100 kDa | Could reflect signal peptide or propeptide cleavage, but its identity requires confirmation |
| Band above ~100 kDa | Could reflect N-linked glycosylation or an uncleaved precursor; neither shift is established here |
| Multiple bands | Could include isoforms 1A and 1B or differently processed forms; their separation is unestablished |
💡Expected DSC1 appearanceDSC1 has a predicted mass of 100 kDa and an empirical band at ~100 kDa; use an appropriate band-identity control when interpreting additional bands.
| UniProt predicted mass | Sets a 100 kDa reference, matching the observed ~100 kDa band |
| N-linked glycosylation at Asn165 | May affect apparent migration; a visible shift is not established |
| N-linked glycosylation at Asn546 | May affect apparent migration; a visible shift is not established |
| Signal peptide and propeptide cleavage | Can make a processed form smaller than its precursor |
| Splice isoforms 1A and 1B | May differ in size; their masses and band separation are not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | DSC1 is a cell-membrane desmosomal protein that may be poorly recovered in the lysate | Check membrane-protein extraction and a DSC1-positive sample |
| Band higher than expected | N-linked glycosylation or incomplete precursor processing is possible | Compare with an N-glycosidase-treated sample and verify band identity |
| Band lower than expected | Signal peptide or propeptide cleavage is possible | Compare with a DSC1-positive control and check the antibody epitope |
| Multiple bands | Isoforms 1A and 1B or different processing states are possible | Use an independent DSC1 antibody or depletion control to identify the bands |
| Weak or no signal | Low recovery of membrane-associated DSC1 is possible | Check extraction, loading, and antibody detection with a positive control |
| Broad smear instead of sharp band | Variable N-linked glycosylation is possible but unconfirmed | Compare treated and untreated samples and confirm DSC1 specificity |
Experiment evidence
Western blot images showing DSC1 band
M03478 · DSC1
M03478 · DSC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for DSC1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for DSC1 in Western blot, you can use hair tissue, which HPA rates as highly positive.
Positive control: Hair (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: As a desmosomal membrane protein, DSC1 may require careful extraction for a strong signal.
HPA tissue expression evidence for DSC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Hair | cells in internal root sheath | High | Protein (IHC) | HPA → |
| Ovary | follicle cells | High | Protein (IHC) | HPA → |
| Skin | cells in corneal layer | High | Protein (IHC) | HPA → |
| Cerebellum | Bergmann glia - cytoplasm/membrane | Medium | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced DSC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for DSC1, answered from its protein features.
How should DSC1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Can DSC1 isoforms produce different bands?
Isoforms · Possibly. Isoform 1B replaces residues 830..840 and lacks residues 841..894 relative to 1A. This sequence difference could affect band position, but the features do not establish that two bands will resolve on a blot.
Check its epitope against both sequences. An antibody targeting residues 841..894 of isoform 1A would miss isoform 1B, which lacks that region. An epitope within the changed 830..840 segment may also behave differently.
Check its epitope against both sequences. An antibody targeting residues 841..894 of isoform 1A would miss isoform 1B, which lacks that region. An epitope within the changed 830..840 segment may also behave differently.
What does DSC1 phosphorylation imply for blot interpretation?
PTM · UniProt lists phosphothreonine at position 385 in the precursor sequence. A phospho-specific antibody must recognize that site; a total DSC1 antibody does not establish its phosphorylation state. Feature presence alone does not demonstrate a visible band shift. Paper or antibody residue numbering may differ from UniProt precursor numbering.
Does this guide establish induction of DSC1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for DSC1 Western blot?
Transfer · DSC1 is an approximately 100 kDa, single-pass membrane protein. Use a transfer setup validated for proteins around 100 kDa, then check transfer efficiency on the membrane and residual protein in the gel. The supplied features do not distinguish wet from semi-dry transfer.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03478 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should DSC1 be quantified across samples?
Quantitation · DSC1 localizes to the cell membrane and desmosomes. Keep extraction and fractionation consistent across samples, compare bands within the linear detection range, and normalize to an appropriate loading measure. If the antibody detects both isoforms, define whether the measurement includes one or both.
Does a 100 kDa DSC1 band match its predicted mass?
Interpretation · Yes. The observed band is approximately 100 kDa, matching the supplied prediction. DSC1 also has a signal peptide (1..29), propeptide (30..134), and N-linked glycosylation sites at Asn165 and Asn546. These features alone do not establish a visible shift or explain a mass difference.
Consider the shorter 1B isoform, processing of the signal peptide and propeptide, and glycosylation at Asn165 and Asn546. Check whether the antibody epitope is retained in each candidate form. These features suggest possibilities; they do not identify an unexpected band without further evidence.
Consider the shorter 1B isoform, processing of the signal peptide and propeptide, and glycosylation at Asn165 and Asn546. Check whether the antibody epitope is retained in each candidate form. These features suggest possibilities; they do not identify an unexpected band without further evidence.
Boster reagents
DSC1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Desmocollin 1 Rabbit Monoclonal Antibody
Cat # M03478
M03478 is a rabbit monoclonal anti-DSC1 antibody listed for human, mouse, and rat. Its Western blot image shows an approximately 100 kDa band in human K562 cell and rat lung lysates. No publication evidence or mouse blot is supplied.
Which to pick: M03478 is the only listed option. Its supplied Western blot image uses human K562 and rat lung lysates at 1:500 under reducing conditions; mouse reactivity is listed but has no shown blot.
Source: BosterBio DSC1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q08554.
- Human Protein Atlas. DSC1 tissue expression.
- PMC10464112 — target-verified WB comparison
- PMC11151536 — target-verified WB comparison
- PMC4999951 — target-verified WB comparison