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- Table of Contents
DSG1 / Desmoglein-1 · Western blot design guide
Design a Western Blot for DSG1
Real validated DSG1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-DSG1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
DSG1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~113.7 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Cervix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated DSG1 Western Blot Protocols
The A02655-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A02655-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | 1:20000 (catalog A02655-1) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected DSG1 Western Blot Band Size?
DSG1 has a predicted precursor mass of 113.7 kDa; cleavage, glycosylation and isoforms could affect migration, but no empirical band size or shift is established.
| Band near 113.7 kDa | compatible with the predicted precursor mass, but identity needs confirmation |
| Band below 113.7 kDa | could reflect signal peptide and propeptide cleavage |
| Band above 113.7 kDa | could reflect N-linked glycosylation; its migration effect is unmeasured |
| Multiple bands | could reflect processing or isoforms 1 and 2; their separation is unestablished |
💡Expected DSG1 appearanceThe UniProt precursor mass is 113.7 kDa; processing and N-linked glycosylation could alter migration, but no empirical band size is supplied, so confirm band identity with antibody and sample controls.
| Predicted precursor mass | 113.7 kDa is the sequence-based reference, not a measured band |
| Signal peptide at residues 1–23 | cleavage could lower mature protein mass relative to precursor |
| Propeptide at residues 24–49 | cleavage could further lower mature protein mass |
| N-linked glycosylation at Asn36 | occurs within the propeptide and would be removed with it |
| N-linked glycosylation at Asn110 and Asn180 | could raise apparent mass; the shift is not quantified |
| Isoforms 1 and 2 | may differ in size, but their masses and band separation are unknown |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | N-linked glycosylation may affect migration | compare untreated and deglycosylated samples with identity controls |
| Band lower than expected | signal peptide or propeptide cleavage may reduce mass | check antibody epitope and compare precursor with processed protein |
| Broad smear instead of sharp band | variable N-linked glycosylation is possible | compare untreated and deglycosylated samples |
| Multiple bands | processing or isoforms 1 and 2 may contribute | compare epitope-specific antibodies and isoform expression controls |
| Weak or no signal | this membrane protein may be poorly recovered from the sample | check membrane extraction and use a positive control |
Experiment evidence
Western blot images showing DSG1 band
A02655-1 · DSG1
M02655-1 · DSG1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for DSG1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for DSG1 in Western blot, you can use cervix tissue, which has high HPA expression.
Positive control: Cervix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: As a desmosomal membrane protein, DSG1 may require efficient membrane protein extraction for a strong signal.
HPA tissue expression evidence for DSG1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Skin | cells in corneal layer | High | Protein (IHC) | HPA → |
| Vagina | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced DSG1 Western Blot Tips
Deeper troubleshooting and optimisation questions for DSG1, answered from its protein features.
How should DSG1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How could DSG1 isoforms affect antibody detection?
Isoforms · UniProt lists isoforms 1 and 2; residues 1–641 are missing in isoform 2. Check whether the antibody epitope falls within that region before expecting it to detect both isoforms. Their band positions cannot be established from these features alone.
Which DSG1 glycosylation sites matter when interpreting bands?
PTM · UniProt lists N-linked sites at Asn36, Asn110, and Asn180. Asn36 falls within the annotated propeptide at residues 24–49, so consider the form of DSG1 your antibody detects. These site annotations alone do not establish a visible band shift.
Yes. UniProt annotates phosphoserine at position 579, using its sequence numbering. Check that numbering when comparing antibody or paper labels. The annotation does not establish that phosphorylation produces a separate Western-blot band.
Yes. UniProt annotates phosphoserine at position 579, using its sequence numbering. Check that numbering when comparing antibody or paper labels. The annotation does not establish that phosphorylation produces a separate Western-blot band.
Does this guide establish induction of DSG1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for DSG1 Western blot?
Transfer · DSG1 is a predicted 113.7 kDa single-pass membrane protein. Validate the chosen transfer method for protein near this size and check transfer before interpreting a weak signal. The supplied features do not specify one transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A02655-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should DSG1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should DSG1 migrate at its predicted 113.7 kDa?
Interpretation · Use 113.7 kDa as a reference, not an expected band position. DSG1 has a signal peptide at residues 1–23, a propeptide at 24–49, and listed N-linked glycosylation sites. These features warrant checking band identity, but they do not establish a visible shift or a specific apparent mass.
DSG1 is annotated at the cell membrane, desmosomes, cytoplasm, and nucleus. Compare the same sample fraction and processing conditions across lanes; a change in the fraction examined can change the measured signal. Check the antibody epitope if isoform 2 may be present.
Consider the annotated signal peptide (1–23), propeptide (24–49), and isoform 2, which lacks residues 1–641. Check the antibody epitope and whether the band appears in the expected sample fraction. These features suggest possibilities; they do not identify an observed band.
UniProt places DSG1 at the cell membrane and desmosomes, and also annotates cytoplasm and nucleus. Select a fraction suited to the DSG1 pool being measured and record that choice when comparing results. A weak signal in one fraction alone does not establish low total DSG1.
DSG1 is annotated at the cell membrane, desmosomes, cytoplasm, and nucleus. Compare the same sample fraction and processing conditions across lanes; a change in the fraction examined can change the measured signal. Check the antibody epitope if isoform 2 may be present.
Consider the annotated signal peptide (1–23), propeptide (24–49), and isoform 2, which lacks residues 1–641. Check the antibody epitope and whether the band appears in the expected sample fraction. These features suggest possibilities; they do not identify an observed band.
UniProt places DSG1 at the cell membrane and desmosomes, and also annotates cytoplasm and nucleus. Select a fraction suited to the DSG1 pool being measured and record that choice when comparing results. A weak signal in one fraction alone does not establish low total DSG1.
Boster reagents
DSG1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Dsg1/Desmoglein 1 Antibody
Cat # A02655-1
Real WB data
Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody
Cat # M02655-1
The catalog reports two anti-DSG1 antibodies with reported Human, Mouse, and Rat reactivity. Each has a Western blot image: A02655-1 uses 293T lysate, while M02655-1 uses human skin lysate. These images document specific tested samples, not validation across every listed species.
Which to pick: For human skin lysate, M02655-1 has the directly relevant Western blot image. A02655-1 has an image using 293T lysate with a 1:1000 primary dilution. Both list Human, Mouse, and Rat reactivity; the supplied images do not establish performance in all those contexts.
Source: BosterBio DSG1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q02413.
- Human Protein Atlas. DSG1 tissue expression.
- PMC6957249 — target-verified WB comparison
- PMC6099613 — target-verified WB comparison